UPLC-MS-MS法测定八宝粥罐头中乙二胺四乙酸二钠残留量.pdfVIP

208 2015， 协1.36， NO.04 ※安全检测 自由融事 UPLC-MS-MS法测定八宝粥罐头中 乙二胶四乙酸二纳残留量 杨长志，韩广源，姜冰，魏冬旭，程阳 (黑龙江出入境检验检疫局，黑龙江哈尔滨 15仪泊1) 摘 要z 建立超高效液相色谱-串联质谱法测定八宝粥罐头中乙二胶四乙酸二铀 C ethylenediaminetetraacetic acid disodium , EDTA-2Na) 残留量的检测和确证方法。试样中残留的EDTA-2Na用水高速匀浆提取，提取液纤，三氯 化铁衍生化、三氯甲烧和MAX阴离子交换柱净化后，用超高效液相色谱·串联质谱仪检测和确证，外标法定量。 2 EDTA-2Na质量浓度在1.0~50.0μg/mL范围内时，线性关系良好. EDTA-2Na的相关系数 CR ) 为0.992 2 。在 20~400 mg/kg添加范围内，样品平均加标回收率在79.4%~ 105.3 9毛之间，相对标准偏差为7.939毛~9.949毛。 EDTA-2Na 的最低检出限为20 mg/kg。该方法具有灵敏、准确、稳定等优点，可用于八宝粥罐头中EDTA-2Na残留量测定。 关键词:乙二肢四乙酸二锅:超高效液相色谱·串联质谱:八宝粥罐头 Determination of Ethylenediaminetetraacetic Acid Disodium Residues in Canned Eight-Ingredient Porridge by UPLC-MS-MS YANG Changzhi, HAN Guangyuan, JIANG Bi吨， WEI Dongxu. CHENG Yang (Heilongjiang En位y and Exit Inspection and Quarantine Bureau. Harbin 15创)()1 ， China) Abstract: An ultra perfo口nance liquid chromatography-tandem mass spectrome町Y (UPLC-MS-MS) method was developed for the determination and confirmation of ethylenediaminetetraacetic acid disodium (EDTA-2Na) residues in canned eight-ingredient porridge. The EDTA-2Na residues in the test samples were extracted with water by means of high叩eed homogenization. The extract was derivatized with FeCl3 reagent , cleaned up by chloroform and MAX anion exchange colurnn.τbe residues were determined and confirmed by UPLC-MS-MS using an extemal standard method. The linear range was 1.0-50.0μ.g/mL for EDTA-2Na with a correlatio