β-(3,4-二羟基苯基)-α(S)-羟基丙酸冰片酯脂肪乳的HPLC法测定.pdfVIP

中国医药工业杂志; Chinese Joumal of Pharmaceuticals 2012, 43 (8) • 697 • ß- (3，4- 二注基苯基)-α (S) - 提基丙酸ì7J 片自旨脂肪乳的 HPLC 法测定 杨凌鉴l ，手i、宇宏1 ，忠民权2，李霞3 ，张亚军* (1.西北大学生命科学学院，陕西西安 710069; 2. 西安力邦制药有限公司，陕西西安 710075; 3. 陕西省食品药品检验所，陕西西安 710061) 摘要z 制备并拆分丹参素冰片酶的立体异构体，得到 ß- (3，4-二垣基苯基 )-α(8) -控基丙酸冰片酶 (1) .建立了高效液相 色谱法测定 1 脂肪乳中的 1. 采用 C 色谱柱，以甲醇-0.2% 甲酸 (65 : 35) 为流动相，检测波长280 nrn. 1 在 10.7 - 18 685μglml 浓度范围内线性关系良好.平均回收率为 100.3%. RSD 为1.34%. 关键词:萨(3，4- 二经基苯基)-(1 (8)-经基丙酸冰片酶z 丹参素冰片酶:脂肪乳:高效液相色谱:测定 中圈分类号: TQ4侃7+2; 0657.7+2 文献标志码:A 文章编号: 1ω1-8255(2012)08-0697-03 Determination ofLipid Emulsion of ß- (3 ,4-Dihydroxyphenyl) -0. (S) -hy的xy Propionic Bomeol Ester by HPLC 1 2 YANG Lingjian , SUN Yuhong , HUI Minquan , LI Xia 3, ZHANG Yajun忡 (1. The College ofLife Sciences. Northwest Universi.职 Xian 710069; 2. Xian Libang Pharmaceutical Ltd.. X ian 710075; 3. Shaanxi 1nstitutefor Food and Drug Control. Xian 710061) ABSTRACT: One of the stereoìsomer毡， ß- (3，4-dihydroxyphenyl) -α($) - hydroxy propionic bomeol ester (1) , was obtained by chiral s叩aration of tanshinol bomeol ester. An HPLC method was established for the determinatation of 1 in its lipid emulsion. A C 18 column was used with the mobile phase of methanol-0.2 % formic acid (65 : 35) at the detection wavelength of 280 nm. The calibration curve w副 linear in the concentration range of 10.7 - 685μg/ml.币le recovery was 100.3% , with RSD of 1.34%. Key Words: 萨(3，4-dihydroxyphenyl) 咀($) -hydroxy propionic borneol ester; tanshinol borneol ester; lipid emulsion; HPLC; determination 丹参素冰片酷 (