β-环糊精增效褪色光度法测定饲料中钴含量.pdfVIP

- 36 中国饲料 2013 年第 9 期 级E皿圆圆眼 β-际糊翁精致涟也先度过训费:饲斜命传令查 淮海工学院化学工程学院 李咏梅 连云港师范高等专科学校 李人字*印园国 [摘要]建立钻( 11 )一硫氨酸馁-亚甲蓝一β一环糊精体系褪色光皮法测定饲料中佑含量。采用微波消解和筑基葡 聚糖凝胶分离技术对饲料进行预处理，测定结果表明:最大褪色波长位于 666 nm. 钻含量在 0-320μg/L 符合比尔 5 定律，检测~为 5.5 μg/L. 表观摩尔吸光系数为1.16X10 L/mol.cm。用该方法测定饲料中钻含量，结果与原子吸收法 一致，相对标准偏差为 0.7% - 1. 4%(n=5). 样品加标回收率为 98.5% - 101.0%。 [关键词]褪色光度法;钻;饲料;测定 [中图分类号] S816.17 [文献标识码] A [文章编号]1∞4-3314(2013)09-0036-03 [Abstract] A new fading spectrophotometric method for determination of cobalt(Co)content in feeds was established. which was based on the Co( 11 )- thiocyanate amine - methylene blue - ß-cyclodextrin system combined with techniques including microwave digestion and speciation of sulphydryl dextran gel for pretreatment of feeds.The results showed that: Beer s law was obeyed for 0 - 320μg/L cobalt wi出 the maximum fading wavelength of 666 nm. The detection limit was 5.5μg/L.咽le appa陀nt molar absorption coefficient was 1. 16xlOS υ(mol.cm).This method was applied ωthe determina- tion of cobalt content in feeds.The results obtained by this method agreed well with those obtained by atomic absorption spectrometry.Relative standard deviation (RSD)was in the range of 0.7% - 1.4% (n=5 ) .and recovery was in the range of 98.51)串- 101.0%. [Key words] fading spectrophotometry; cobalt ;feed ;determination 离样品预处理技术，建立了饲料中钻含量测定的 钻是动物的必需微量元素之一，是维生素 B 12 的组成成分。钻具有促进消化和生长，调节造血机 钻( n)-硫氨酸镀-亚甲蓝-β-环糊精体系褪色光 能和糖代谢，增强繁殖能力和免疫机能等生理功 度法