分光光度法测定青钱柳叶中总三萜皂甙含量.pdfVIP

第 29 楼第 3 期 江西农业大 报 Vol. 29 ,No. 3 2007 年6 月 Acta Agriculturae Universitatis Jiangxiensis June ,2007 文章编哥: 1000 - 2286 (2007) 03 - 0449 - 05 分光光度法测定青钱柳叶中总三船主运前含最 徐睿庸，洪艳平，上官新晨\尹忠平，蒋艳，陈木森 (江西农业大学食品科学与工程学院，植物资源开发与利用实验室，江西南昌 330045 ) 摘要:以分光光度法定震，优化香尊醒-高氯酸法的黑色条件:50 glL 香草隆『冰乙酸与高氯酸分别为 0.3 roL 利。.6 roL ,80 c水浴 10 roin ，测定波长 550 nro 。以人参皂试 Rb. 为对照品，绘制标准曲线，回归方程 m= 0.1627A+0.0001 ， 相关系数 r =0. 9996 结果显示，分光光度法测寇背钱柳叶中总宝路皂试含擎的精密度、 0 RSD 分别为 2.28% 、2.42% 、1. 99% 。 加样回收率、稳定性试验 关键诩:青钱柳;总二三阳皂战;分光光度法;优选;测定 中图分类号:Q94 -331 ,TSI0 1. 3 丁 文献标识码:A Determination of Total Triterpene Saponins in Cyclocarya paliurus (Batal. ) Iljinskaja by Spectrophotometry XU Rui …yong , HONG Yan 叩 ping ， SHANGGUAN Xin …chen * , YIN Zhong - ping , JIANG Yan , CHEN Mu - sen (College of Food Science and Engineering ,Plant Resources Exploitation and Utilization Laborato巧， JAU ， Nanchang 330045 ,China) Abstract: The content of total triterpene saponins was determined by spectrophotometry , the optimal con- dition was determined in the vanillin - perchloricacid method: O. 3 mL of 50 g/L vanillin in acetic solution and 0.6 mL of perchloricacid was added , then the mixture was warmed up in a waterbath at 80 t: for 10 min. and the d咽tection wavelenth was 550 nm. And the ginsenoside Rb 1 standard sample was usded to make standard curve , the regressive equation was m =0. 162 7A +0.000 1,the correlation coefficient was 0.9996. The ex- perimental result showed the relative standard deviations (RSD) of the accuracy ,recoveries and stability were 2.28% ,2.42% and 1. 99% ,respectively.