饲料中维生素和无机盐对rBmNPV-Bm表达系统植酸酶基因表达活性的影响.pdfVIP

昆虫 学 报 Acta Entomologica Sinica，June 2008，51(6)：575—579 ISSN 0454—6296 Effects of vitamins and inorganic salt in artificial diet on the expression of phytase gene in rBmNPV-Bm expression system SHANG Jin—Yan 一，WANG Bing ，CUI Wei—Zheng ’， WU Xiao—Feng ，ZHANG Zhi—Fang4 (1．College of Forestry，Shandong Agrimdtural University，Tai’an，Shandong 271018，China；2．Shandong FoodDrug Vocational College，Weihai，Shandong 264210，China；3．College of Animal Sciences，Zhejiang University，Hangzhou 310029，China； 4．hlstitute of Biotechnology．Chinese Academy of Agricuhural Sciences，Beijing 100081，China) Abstract：【Aini】Enhancing the yield of silkworni bioreactor and disclosing the relation between the host nutritive condition and the expression of foreign gene in rBmNPV—Bm systen1．【Methods】The effects of vitamins and inorganic salt in artifieial diet for the host inseet of baculmtirus．silkworm Bombyx rnori．on the acti~rit) of foreign gene products was studied using BnINPV as an expression vector and phytase as a reporter gene．【Results】The activity of phytase in silkworni haenio1) mph varied reniarkably with the aniount of added vitaniin and inorganic salt．The phytase activity in silkworni haeniolymph reached the 1naxiIllUIll when the aniount of vitaniin C was 1％ of the dry weight of artificial diet． vitaniin B niixture 0．25％ ，inorganic salts niixture 1％ and the anlount of vitaniin B6 is 2 rug per 1 00 g dry niatter weight，respectively in the designed test scope．【Conclusions】The expression of phytase gene in BnINPV—Bni expression systenl could be enhanced efficientlyby altering the levels of nlicronutrients available in the artificial diet for silkworni lanrae． Key words：BnINPV—Bni expression s)rsteni；vitaniin；inorganic salt；phytase gene；expressi