大肠癌患者血清中BCRP表达及化疗疗效关系.doc

大肠癌患者血清中BCRP表达及化疗疗效关系【摘要】目的：观察大肠癌中乳腺癌蛋白（BCRP）的表达与化疗疗效的关系。方法：抽取56例大肠癌患者和40例健康成人静脉血，采用流式细胞术（FCM）法，BCRP单克隆抗体一抗，FITC标记为二抗，同时以健康成人标本为对照，检测患者及健康人血清中BCRP表达，追踪疗效，比较BCRP在患者及健康人中的差异及缓解者及耐药者中BCRP差异，分析BCRP与大肠癌化疗疗效关系。结果：FCM检测结果示：肿瘤患者血清BCRP[C期（2.25±1.02）mg/L，D期（2.93±1.32）mg/L]；健康人血清BCRP（0.48±0.01）mg/L，大肠癌患者血清中BCRP表达高于健康人，化疗耐药组与缓解组BCRP表达分别为（2.97±1.38，1.65±0.75）mg/L，耐药组明显高于缓解组，也高于正常成人，化疗敏感者与健康人差异无显著统计学意义（P0.05）。结论：BCRP在大肠癌患者血清中高表达，其表达强度与5-FU为主的化疗敏感性有关。 【关键词】大肠肿瘤；耐药蛋白；化疗敏感性 文章编号：1009-5519（2008）01-0027-03 中图分类号：R73 文献标识码：A Relationship between expression of serum BCRP and curative effect of chemotherapy in patients with large intestine cancer WANG Feng-lian1，YANG Ya-ming2 （1.Department of Oncology,The Fourth People’s Hospital of Ruzhou，Henan 467500，China；2.Institute of Anti-parasitic Diseases of Yunnan/Institute of Pathogeny and Biological Vector，Dali College,Yunnan 665000，China） 【Abstract】Objective：To observe the relationship between expression of serum BCRP and curative effect of chemotherapy in patients with large intestine cancer.Methods：By FCM，BCRP monoclonal antibody as the first antibody，FITC marker as the second antibody and the health person’s sample as comparison，the venous blood was taken out in 56 patients with large intestine cancer and 40 health persons to detect the expression of serum BCRP and track the curative effect.The difference of BCRP in patients and health peron as well as reliever and resistancer was compared respectively and the relationship between expression of serum BCRP and curative effect of chemotherapy in patients with large intestine cancer was analysed.Results：FCM suggested：BCRP in patients with large intestine cancer was 2.25±1.02 in C period and 2.93±1.32 in D period，which was 0.48±0.01 in health person.The expression of BCRP in patients with large intestine cancer was higher than that in health person.The expression of BCRP was 2.97±1.38 in the resistancer group and 1.65±0.75 in the reliever group，the former was higher than the