蜜蜂主要变应原酸性磷酸酯酶基因的克隆及原核表达载体的 - 深圳大学.pdf

中 国 病 原 生 物 学 杂 志 2010 6 ! 5 6 J ournal of Pathog en Biology ! Ju n 2010, ! Vol. 5, No. 6 40 1 * 1 2 3 3 3* * 3* * 张强, 叶卫翔 , 刘志刚 , 陈思 , 罗新萍 , 黄钟 ( 1. , , 518060; 2. , , 518067; 3. , 518060) ! ! Ap is cerana cerana ( Api m3) , ! , RNA RTPCR , PET28a ! ! Api m3 , 1 167 bp, 388 , 45. 279 9 ku, 5. 53G n Bank 97% ! ! Apim3 , ! ; ; ; ; ! Q966 ! ! ! ! A ! ! ! ! 20 10) 0640104 [ Journal of Pathogen B iology . 20 10 Jun; 5( 6) : 40 1- 404. ] Molecule cloning of amajor allergen acid phosphatase(Apim3) gene fromhoneybees and construction of aprokaryoticexpression vector 1 2 3 3 3 3 ZHANG Qiang , YE W ix iang , LIU Zhigang , CHEN Shi , LU O Xinping , HU ANG Zhong ( 1.A llergy and Immunology Institute, School of Medicine, Shenzhen University , Shenz hen 518060, uangdong, Chi na; 2. Food Quarantine Technology , ShenzhenEntry- exit I nsp ection and Quarantine Bureau; 3.ShenZhen University School of Med ical) Abstract ! Objective! T o clon a major all rg n acid phosphat as ( Apim3) g n from hon yb s ( Ap is cerana cerana) and construct a prokaryotic xpr ssion v ctor. ! Methods! Sp cial prim rs w r d sign d on th basis of th r port d g n . T otal RNA w as xtract d from b s. RTPCR was us d to am plify th Apim3 g n and s qu nc analysis w as p r form d. Fragm nts w r subclon d into th xpr ssion v ct or pET28a. ! Results! Th A pim3 g n w as obtain d from hon yb s and a prokaryotic xpr ssion v ctor w as construct d.