促红细胞生成素与炎性细胞因子在脑瘫发病中的作用.pdfVIP

· 62 · 中国康复理论与实践2008年1月第l4卷第1期Chin J Rehabil Theor ct!』 ! ! !l4 1 堡 · 临床研究· 促红细胞生成素与炎性细胞因子在脑瘫发病中的作用 陶维元 ，文芳 ，姚汉云 ，孙杨 [摘要] 目的 探讨脑保护因子促红细胞生成素(EP0)与免疫炎性细胞因子在脑瘫发病中的作用。方法 检测31例脑瘫患儿 (脑瘫组)和2O名健康儿童(脑瘫对照组)及37例脑瘫高危因素新生儿(新生儿病例组)和2O名正常新生儿(新生儿对照组)的血清 EPO、肿瘤坏死因子(TNF)a和白细胞介素(IL)一6水平。结果 新生儿病例组血清EPO水平明显高于新生儿对照组和脑瘫组(P o．o1)，脑瘫组血清EPO水平与脑瘫对照组比较无显著性差异(Po．05)；新生儿病例组血清TNF-~和 IL一6水平明显高于新生儿 对照组(Po．01)；脑瘫组血清 TNF-a和IL一6水平明显高于脑瘫对照组(Po．01)，脑瘫组血清TNF- 水平高于新生儿病例组(P o．05)，但两者间IL一6水平无显著性差异(Po．05)。结论 炎性细胞因子介导的脑破坏作用在与EPO的神经保护作用的抗衡 中占据优势，促进或导致脑瘫的发生。 [关键词]脑性瘫痪；促红细胞生成素；炎性细胞因子；肿瘤坏死因子(TNF)；白细胞介素(IL)；免疫一神经一内分泌网络 Influence of Erythropoietin and Inflammatory Cytokines on Pathogenesis of Cerebral Palsy 丁AO Wei yuan，WEN Fang，yA0 Han yun，et a1．Department of Neurology，Peoplgs Hospital，Wuhan University，Wuhan 430060，Hubei，China Abstract： Objective To investigate the effect of erythropoietin(EPO)as a brain—protective factor and inflammatory eytokines in the pathogenesis of cerebral palsy(CP)．Methods Serum samples from 31 CP patients，37 neonates with CP risk factors such as hypoxic-ischemic inj ury and／or perinatal infection，and 20 controls of neonates or children were obtained respectively．EPO，tumor necrosis factor(TNF)一a and interleukin(IL) 6 levels were measured with the enzyme—linked immunosorbent assay double sandwich method(ABC—ELISA)．Results The serum EPO level of neonatal patients was higher than that of controls or CP group(PO．01)， but there was no significant difference between CP group and controls．The serum TNF—a and IL一6 levels of CP and neonatal patients were higher than that in controls(P0．01)．The serum TNF-a level of CP group was higher than that of neonatal patients(P 0．05)．There was no significant difference between CP group and neonatal patients in serum IL一6 leve1．Conclusion The inflammato— ry respo