十全大补汤对Lewis肺癌小鼠MMP-9基因表达影响.doc

十全大补汤对Lewis肺癌小鼠MMP-9基因表达影响摘 要：目的：探讨十全大补汤对Lewis肺癌组织中MMP-9基因表达的影响。方法：50只小鼠采用细胞株接种的方法复制Lewis肺癌转移模型，造模成功后，将小鼠分为模型组，CTX组，十全大补汤低、中、高剂量组。采用RT-PCR方法检测十全大补汤对 Lewis肺癌组织中MMP-9基因表达的影响。结果：十全大补汤中、高剂量组的肿瘤转移抑制率为43.20%、29.59%，两组中MMP-9的表达下降，与模型组比较有统计学意义（P＜001）。结论：十全大补汤下调MMP-9基因表达是其抑制肺癌转移的分子机制之一。 关键词：肺癌；十全大补汤；基质金属蛋白酶-9 中图分类号：R285.5文献标识码：A文章编号：1673-7717(2011)12-2767-03 Effect of Shiquan Dabu Decoction on MMP-9 gene Espression in Lewis Lung Cancer Mice SUN Daxing1，ZHANG Yongjun2, BAO Wenlong2 (1.Zhejiang Tonglide Hospital, Hangzhou 310012,Zhejiang,China; 2.Zhejiang Cancer Hospital, Hangzhou 310022,Zhejiang,China) Abstract:Objective: To study the effect of Shiquan Dabu Decoction on MMP-9 gene expression in Lewis lung cancer mice. Methods: The 50 mice were randomly divided into 5 groups: the model group, the Cyclophosphamide group, the low、middle and high dosage groups of Shiquan Dabu Decoction after the cancerometastasis mice model of Lewis lung cancer were established by inoculating tumor cell. The gene expression of MMP-9 in tumour tissues were detected by reverse-transcription polymerase chain reaction(RT-PCR). Results: The tumor metastasis inhibition rate in the middle and high dosage groups of Shiquan Dabu Decoction were 43.20% and 29.59% respectively. Moreover, the contents of MMP-9 genes,compared with model group, were decreased (P＜0.01). Conclusion: Reducing gene expression of MMP-9 was one of molecular mechanism of Shiquan Dabu Decoction inhibiting lung cancer’s metastasis. Key words:lung cancer; Shiquan Dabu Decoction; matrix metalloproteinases-9 ________________________________________ 收稿日期：2011-07-22 作者简介：孙大兴（1958-），男，浙江绍兴人，主任医师，硕士研究生导师，研究方向：中西医结合肿瘤临床。祖国医学认为，肺癌自始至终表现为一系列正气为癌毒所耗散的证候，癌毒的发生与发展均本于正虚，且随着病情进展，正气不断耗散，导致其外抗和内固癌毒的能力下解，进而发生癌毒的扩散。据此，我们选用经典的扶正固本方剂—十全大补汤进行抗肿瘤转移的研究，并对其作用机理作一初探，为临床应用提供理论依据。 1 材料与方法 1.1 实验动物及瘤株 C57BL/6小鼠50只，体重（20±2）g，鼠龄4周，雌雄各半。购于上海医学科学院实验动物中心。小鼠Lewis肺癌瘤株，购于上海医学科学院实验动物中心。瘤株于浙江中医药大学动物中心进行传代。 1.2 药物 十全大补汤：黄