益气补肾口服液体外抗肿瘤作用实验探究.docVIP

益气补肾口服液体外抗肿瘤作用实验探究摘 要：目的：研究益气补肾口服液的体外对SMMC-27721细胞及A-549细胞生长抑制作用。方法：采用活细胞计数法及MTT法观察益气补肾口服液对上述两种人瘤细胞进行检测和观察。结果：益气补肾口服液对上述两种人瘤细胞的生长增殖曲线具有明显的抑制作用。对两种人瘤细胞降解MTT半数抑瘤浓度(IC50)分别为1.02mg/mL、0.73mg/mL；对两种人瘤细胞的集落形成有明显的抑制作用，最大抑制率分别为78.48%、89.17%。结论：益气补肾口服液体外具有明显的抗肿瘤活性。 ? 关键词：益气补肾口服液；SMMC-27721细胞 ；A-549细胞；生长抑制? 中图分类号：R273 文献标识码：A文章编号： 1673-7717（2008）11-2445-02 An Experimental Study on Anti-tumor Effects of Yiqi Bushen Liquid in Vitro ?? RUAN Ye?ping, HU Xiu?min?（Zhejiang Chinese Medical University,Hangzhou 310053,Zhejiang,China ）?? Abstract：Objective：To investigate the anti-tumor effects of Yiqi Bushen liquid on homo sapien hepatoma carcinoma cells, SMMC-27721, and homo sapien lung carcinoma cells, A-549 in vitro.Methods：Viable cells count，MTT staining assay were used to assess the anti-tumor effects of Yiqi Bushen liquid on the two kind cells. Results：Yiqi Bushen liquid had a certain inhibitory action on growth curve of SMMC27721 and A549 cells.The IC50of the two cells were 1.02mg/mL and 0.73mg/mL respectively. It also could inhibit the two cells forming colonies. The highest inhibitory rate of Yiqibushen liquid against SMMC27721 and A549cells were 78.48% and 89.17% respectively.Conclusion：Yiqi Bushen liquid have strong anti-tumor effects in vitro.? Key words：Yiqi Bushen liquid;SMMC-27721 cells;A-549 cells;growth inhibiting 益气补肾口服液根据肿瘤手术、化疗、放疗后正气亏虚之病机特点而组方，由黄芪、茯苓、女贞子、枸杞子、猪苓、莪术、半枝莲等组成，具有补气固表、滋补肝肾、利水渗湿、活血止痛、败毒抗癌等功效。笔者已经研究表明，益气补肾口服液具有较好的体内抗肿瘤作用（另文报道），现对其体外抗肿瘤作用的实验研究报道如下，为临床用药提供实验依据。? 1 实验材料? 细胞株：人肝癌SMMC-27721细胞株、人肺癌A-549细胞株均由浙江大学医学院提供提供。益气补肾口服液的处方：黄芪20g，茯苓12g，女贞子12g，枸杞子12g，猪苓12g，莪术10g，半枝莲30g，藤梨根30g，生米仁30g，八月札10g，红枣30g，炙甘草6g。将药放入锅中浸泡1h，加水煎煮1h，滤出药液，再加水煎煮30min，过滤，合并药液，浓缩药液分别至4.28g/mL、2.14g/mL、1.07g/mL3个浓度，分别作为高、中、低3个剂量组的给药浓度。MTT试剂(美国Sigma)，RPMI1640细胞培养液(日本日水制药株式会社)，小牛血清购于杭州四季青生物工程材料研究所，批倒置显微(Nikon808434)，CO2培养箱(日本Forma)，酶标仪（Lab systems Dragon wells can MK3)。? 2 方法及观测指标? 2.1 益气补肾口服液对两种瘤细胞生长曲线的影响[1] ①细胞培养：5％CO2培养箱，用含10%小牛血清及青、