硒及丹参酮IIA磺酸钠对兔心肌缺血再灌注损伤血浆NO SOD GSH-Px MDA影响.docVIP

硒及丹参酮IIA磺酸钠对兔心肌缺血再灌注损伤血浆NO SOD GSH-Px MDA影响摘 要：目的：观察硒与丹参酮IIA磺酸钠合用对家兔心肌缺血再灌注损伤血浆NO、SOD、GSH-Px、MDA的影响。方法：将26只家兔随机分成4组，即假手术组、缺血再灌注组、丹参酮IIA磺酸钠组(DS-201)、硒+DS-201组。分别检测血浆中SOD、GSH-Px活力和NO、CK、LDH、MDA含量。结果：DS-201组、硒+DS-201组与缺血再灌注组相比，血浆CK、LDH和MDA含量均显著降低(P0.01)，硒+DS-201组与DS-201组相比，血浆CK、LDH和MDA含量显著降低(P0.01，P0.05)。DS-201组、硒+DS-201组与缺血再灌注组相比，血浆T-SOD活力均显著升高(P0.05)，但硒+DS-201组与DS-201组相比，血浆T-SOD活力没有显著性差异。DS-201组与缺血再灌注组相比，血浆GSH-Px活力无显著性差异。但硒+DS-201组与缺血再灌注组(P0.01)、DS-201组(P0.05)及假手术组(P0.05)相比，血浆GSH-Px活力均显著升高。结论：硒与丹参酮IIA磺酸钠合用对家兔心肌缺血再灌注损伤具有明显的保护作用，并且优于丹参酮IIA磺酸钠。其作用机制主要与提高血浆SOD、GSH-Px活性，清除自由基，抑制脂质过氧化反应有关。 关键词：硒；丹参酮IIA磺酸钠；缺血再灌注损伤 中图分类号：R285.5 文献标识码：A 文章编号：1673-7717(2008)03-0634-03 Influences of Selenium and Sodium Tanshinone IIA on NO，CK，LDH Content and SOD，GSH-Px Activity in Plasma of Myocardial Ischemia Reperfusion Injury in Rabbits WANG Hui1，ZHANG Zhong-lin2，MA Yue-jiao2，LI Yi2，SONG Tian-yi2 (1.Department of Pharmacology，Liaoning University of TCM，Dialian 116600，Liaoning，China； 2.Professional Technical College，Liaoning University of TCM，Shenyang 110032，Liaoning，China) Abstract：Objective：To study the Influences of Selenium and Sodium Tanshinone IIA on NO，CK，LDH content and SOD，GSH-Px activity in plasma of myocardial ischemia reperfusion injury in rabbits. Methods：Twenty-six rabbits were randomly divided into four groups，namely non-operation group，ischemia reperfusion group (IRI)，Sodium Tanshinone IIA group(DS-201)，Selenium and DS-201group.The blood was collected at 60 min reperfusion for determination of NO，CK，LDH content and SOD，GSH-Px activity . Results：In DS-201group and Selenium and DS-201group plasma LDH，CK and MDA content were significantly lower than group IRI(P0.01). In Selenium and DS-201group plasma LDH，CK and MDA content were significantly lower than group DS-201(P0.01，P0.05). In DS-201group and Selenium and DS-201group the activity of SOD was significantly higher than group IRI(P0.05).But there was no significant difference of SOD activity in pla