黏细菌及其次级代谢物生物学性质初步探究.docVIP

黏细菌及其次级代谢物生物学性质初步探究摘要：目的研究分离到的黏细菌So ce shu-1及其次级代谢物的生物学特性。方法用烘干法和DNS法测定So ce shu-1利用纤维素的能力，用滤纸片法和溴化四唑蓝（MTT）法测定So ce shu-1利用纤维素次级代谢物抑制细菌和肿瘤细胞的效果。结果So ce shu-1能利用玉米秸秆和滤纸等纤维素，纤维素酶活性为12.30 UFPA/mL, 其次级代谢物有广谱抗菌活性，对 K562等肿瘤细胞有较好的抑制效果。结论So ce shu-1在能源利用方面，其次级代谢物在药物和食品保鲜方面具有良好的开发前景。 关键词：黏细菌；次生代谢物；生物活性 中图分类号：Q55文献标识码：A文章编号：1672-979X(2009)01-0027-04 Preliminary Study on Bioactivity of Myxobacterium So ce shu-1 and Its Secondary Metabolites LI Yan-li1, ZHANG Yin-lei2, LIU Ying, MA Zhong-liang2* (1. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, China; 2. Shanghai Key Laboratory of Bioenergy Crops, Shanghai University, Shanghai 200444, China) Abstract：Objective To study the bioactivity of myxobacterium So ce shu-1 and its secondary metabolites which can be separately obtained. Methods The oven-drying method and DNS method were used to determine the cellulose-utilizing capability of So ce shu-1. The antibacterial and antitumor effects of the secondary metabolites of So ce shu-1 were investigated by filter paper method and MTT method, respectively. Results So ce shu-1 could use the cellulose from cornstalk and filter paper, and its cellulase activity was 12.30 UFPA/mL. The secondary metabolites of So ce shu-1 had broad-spectrum antimicrobial activity and could effectively inhibit the growth of tumor cells, especially inhibiting K562 cell line. Conclusion So ce shu-1 can be used in energy development and its secondary metabolites have a bright development perspective in drug and food fresh-keeping. Key words：myxobacterium; secondary metabolite; bioactivity 目前已从黏细菌中发现约600多种生物活性物质，约占微生物来源总数的5 %。黏细菌产生的生物活性物质种类繁多，结构新颖，作用层次多，作用机制多样等。这些次级代谢物具有抗菌、抗病毒、抗肿瘤、免疫抑制、抗高血压、抗糖尿病、抗疟疾以及抗高胆固醇症等特性。抗生素产生菌比例高于放线菌，现已成为生物活性物质的新微生物来源[1,2]，如epo系列的抗肿瘤药物，比传统抗肿瘤药物紫杉醇具有更多的优点[3]。某些黏细菌能利用纤维素，为将纤维素（农作物废弃物的主要成分）转化为生物燃料提供了新的选择。因此，黏细菌在能源综合利用及其次级代谢物在食品、医药工业的应用上都值得深入研究。我们现从这两方面对分离到的黏细菌进行了初步研究。 1材料与方法 1.1材料 1.1.1菌株和细胞黏细菌：So ce shu-1 按中国药科大学生