输尿管部分梗阻后平滑肌细胞中游离Ca2+浓度改变.docVIP

输尿管部分梗阻后平滑肌细胞中游离Ca2+浓度改变[摘要] 目的：测定大鼠单侧部分输尿管梗阻后输尿管平滑肌细胞中游离Ca2+浓度改变。方法：将Wistar雄性大鼠80只随机分为4组：8周PUUO组20只，16周PUUO组20只，同期对照组各20只。PUUO组采用将大鼠左侧上1\2段输尿管包埋入腰大肌造成单侧输尿管部分梗阻的动物模型，对照组仅分离左侧输尿管。成模后分别于8周和16周分离取出输尿管，分离单个输尿管平滑肌细胞应用Fura-2/AM荧光探针负载检测输尿管平滑肌细胞中游离Ca2+的浓度。结果：输尿管平滑肌静态细胞内Ca2+荧光比值测定结果显示：8周的PUUO大鼠组与对照组大鼠之间细胞内游离Ca2+浓度的差异无统计学意义，而16周的PUUO大鼠组细胞内游离Ca2+的浓度显著升高，且差异具有统计学意义（P＜0.05）。结论：细胞内钙超载是输尿管梗阻后平滑肌功能损害病理过程的重要原因，但是发生较晚，可能在输尿管部分梗阻后期起着重要作用。 [关键词] 单侧输尿管部分梗阻（PUUO）；输尿管平滑肌；细胞内钙离子 [中图分类号] Q81699 [文献标识码]A [文章编号]1673-7210（2011）03（b）-006-03 Changes of cytosol-free calcium concentration in smooth muscle in partial unilateral ureteral obstruction rats YIN Nan, RU Feng, WANG Dongwen* Department of Urology, the First Hospital, Shanxi Medical University, Shanxin Province, Taiyuan 030001, China [Abstract] Objective: To observe the changes of cytosol-free calcium concentration in smooth muscle in partial unilateral ureteral obstruction rats. Methods: Eighty wistar rats had been divided into four groups by random: 8-week experimental group (n=20) and sham control group (n=20); 16-week experimental group (n=20) and sham control group (n=20). Experimental animals left ureters (n=40) had been embedded the upper half into the psoas muscles. Sham control rats only received laparotomy after the model arc established the rats were separated in different experimental point to carry out the intracellular calcium concentration were measured in single ureteral smooth muscle cell in rats using the fluorescent calcium indicator dye Fura-2/AM at rest situation. Results: The intracellular calcium concentration in single ureteral smooth muscle cell in PUUO rats had no significant difference in the initial stage at rest situation in comparison to sham control group [(200.42±36.34) vs (187.63±23.51)]. However, at 16th week after onest, the intracellular calcium concentration in single ureteral smooth muscle cell (454.92±31.69) was significantly increased in PUUO rats as compared with that in sham contr