固相微萃取-气质联用法分析浅裂鳞毛蕨挥发性成分.pdfVIP

www. zgys. org 研究拾文 固相微萃取·气质联用法分析浅裂鳞毛藏挥发性成分 姬志强 张建民 张新民石磊 (解放军第 155 中心医院河南开封 475∞3) 摘 要 目的:研究浅裂鳞毛痪的挥发性成分。方法:采用气相色谱-质谱联用技术首次对浅裂鳞毛族的挥发性成分进行分 析和鉴定。结果:从浅裂鳞毛葳根中鉴定了 28 个化合物，占根中挥发性成分的86.840毛，从叶中鉴定了 24 个化合物，占叶中 挥发性成分的82.79% 。结论:正圣醒有可能是浅裂鳞毛族叶中的主要香气成分，丁美E基甲苯和石竹烯有可能是浅裂鳞毛族 中的药效成分。 关键词 浅裂鳞毛族;固相微萃取;气相色谱-质谱 中固分类号:R284.1 文献标识码:A 文章编号:1∞8-049X( 2015 )07-1120.()3 Analysis of Vola甜e Constituents in Dη， opteris Sublaeta by Head-space Solid Micro-extraction Coupled with GC-MS Ji Zhiqiang , Zhang Jianmin , Zhang Xinmin , Shi Lei (四e 155th Central Hospital of PLA , Henan Kaifeng 475∞3 ， China) ABSTRACT Objective: To investigate the chemical components in the volatile constituents from Dryopteris sublaeta. Methods; The volatile constituents from leaves and roots of D. sublaeta were analyzed by head-space solid micro-extraction coupled with GC-MS for 出e first time. Results: Twenty-eight compounds were identified from the roots , which accounted for 86. 84% of the total volatile con- stituents in the roots of D. Sublaeta. And twenty-four compounds were identified from the leaves , which accounted for 82. 79% of the total volatile constituents in the leaves. Conclusion: Nonanal may be the main fragrant constituent , while butylated hydroxytoluene and caryophyllene may be the active compounds in D. sublaetα. KEY WORDS Dryopteris sublaeta; Solid micro-extraction; GC-MS 浅裂鳞毛藏(Dryopteris sublaeta) 属于鳞毛藏科 立即取出，在气相色谱仪进样口 (250t) ，脱附 1 的鳞毛藏属植物[l] ，在河南伏牛山区作为贯众人 mm。 2.2 色谱与质谱条件 药，临床用于治疗感冒发热、湿热斑菇、腮腺炎等疾 色谱条件: HPδMS 石英弹性毛细管柱 病。化学成分研究发现浅裂鳞毛藏中含有二氢黄 酣、苯乙烯昔等多种化合物[2· ，前期的研究表明， (30.0 m x 250μm， 0.25μm) ;载气为高纯氮气 (Z) -9-十八碳烯酸是浅裂鳞毛藏脂溶性成分中的药 (99.999