ectopic expression of mir-125a inhibits the proliferation and metastasis of hepatocellular carcinoma by targeting mmp11 and vegfmir - 125 a的异位表达抑制肝细胞癌的增殖和转移目标mmp11和vegf.pdfVIP

Ectopic Expression of MiR-125a Inhibits the Proliferation and Metastasis of Hepatocellular Carcinoma by Targeting MMP11 and VEGF 1. 1. 1. 1 1 1 1 1 Qian Bi , Shanhong Tang , Lin Xia , Rui Du , Rui Fan , Liucun Gao , Jiang Jin , Shuhui Liang , 1 1 1 1 1,2 1 1 Zheng Chen , Guanghui Xu , Yongzhan Nie , Kaichun Wu , Jie Liu , Yongquan Shi *, Jie Ding *, Daiming Fan1 1 State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi’an, P.R. China, 2 Department of Digestive Diseases, Huashan Hospital, Fudan University, Shanghai, China Abstract Background: Studies have been shown that miR-125a plays an important role in carcinogenesis, however, the role of miR- 125a in hepatocellular carcinoma (HCC) remains elusive. Methodology/Principal: Real time-PCR (qRT-PCR) was performed to test the significance of miR-125a in HCC. Ectopic expression of miR-125a was used to test the influences of miR-125a on proliferation and metastasis of HCC cells in vitro and in vivo. Predicted target genes of miR-125a were determined by dual-luciferase reporting, qRT-PCR, and western blot (WB) analyses. Then immunohistochemical staining (IHC) was used to detect the expression of target genes, and the correlations and prognostic values of miR-125a and its target genes were also investigated. Conclusions/Significance: Decreased miR-125a was observed in both HCC tissues and cell lines, and associated with patients’ aggressive pathologic features. Up-regulating miR-125a significantly inhibi