糖基化终末产物对大鼠心脏微血管内皮细胞NF-κB和COX-2表达的影响.pdfVIP

intofive 100，200mg／L)thenacting24h．Grouptwo，dividedgroups，normal DMEMculturedwith thesaineconcentra— control(serum—fleeliquidBSA)and tion BSA—AGE(100mg／L)in,iaryingstimulating and ofNF—KBCOX一2werestudiedWesternBlot． proteinexpression by Resuits The resultshadshown treatmentwithBSA—AGEin experiment that，after of and in— NF—KBCOX一2 varyingconcentrations，theproteinexpression creasedina withcontrolled dose—dependentmanner．Comparedgroup，the ofNF—KBwas 4．25一folds proteinexpression 1．99一，2．76一，3．57一，and ofCOX-2was protein 2．39一， respectively(n=5，P0．05)；theexpression 3．41一，4．49一，5．34一fold aftertreat— wit}lAGEs concentrationbutat time ment ofthesalne time．withthe varying of andCOX一2 inatime NF—KB increased prolonged，theproteinexpression with controlled of —dependentmanner．Comparedgroup，theprotein expression NF—KBwas 4．14一folds 1．83一，2．66一，3．52一，and ofCOX一2was 0．05)；theproteinexpression 2．18一，3．21一，4．33一，4． 99一folds respectively(n=5，P0．05)．