NO-1886对高脂%2f高糖%2f高胆固醇饲养小型猪炎症相关因子表达影响.pdfVIP

脏、动脉、骨骼肌等组织炎症因子CRP、IL-6、TNF-、VCAM-1、IL-1beta 蛋白表 达增加。在高脂/高糖/高胆固醇饲料中添加 1.0% NO-1886 后，巴马小型猪空腹血糖、 胰岛素、TG、FFA 浓度均无明显升高；NO-1886 降低了脂肪组织中大部分炎症因 子的表达，其中有 24 种炎症因子水平降低显著，NO-1886 抑制了肝脏、动脉、骨 骼肌等组织炎症因子CRP、IL-6、TNF-、VCAM-1、IL-1beta 的表达增加，减轻肝 脏和动脉组织中炎症细胞浸润；抑制动脉粥样硬化病变发展，改善糖代谢。 结论： 1、高脂/高糖/高胆固醇饮食可以在小型猪诱发糖尿病合并动脉粥样硬化； 2、高脂/高糖/高胆固醇饮食可诱导小型猪肥胖和脂质异位蓄积； 3、高脂/高糖/高胆固醇饮食可明显升高炎症相关因子在小型猪脂肪、肝脏、骨骼肌、 主动脉组织的表达，引起肝脏和动脉组织中的炎症细胞浸润增加； 4、NO-1886 可减轻小型猪肥胖，抑制脂质异位蓄积； 5、NONO -1886 可明显降低炎症相关因子在小型猪脂肪、肝脏、骨骼肌、主动脉组织 的表达，减轻肝脏和动脉组织中炎症细胞浸润。 关键词：NO- 5 ©KdBÆ Effects of NO-1886 on inflammation-associated factors in high-fat/high-sucrose/high-cholesterol diet-fed miniature pigs Abstract Objective: To observe effects of weight gain, ectopic lipid deposition, expression levels of pro-inflammation cytokines in Guangxi Bama-miniature pigs fed by high-fat/high-sucrose/high-cholesterol diet (HFSCD); and to study whether the long-term administration of the LPL activator, NO-1886, inhibit obesity, decrease weight gain, lower the expression of inflammation association cytokines, and alleviate inflammation status，thus protecting against the development of atherosclerosis and diabetes mellitus. Methods: 1) Animal preparation and treatment: Fifteen male Chinese Bama-miniature pigs were randomized into three groups with similar body weight [n=5 in the normal control diet (CD) group; n=5 in the high-fat/high-sucrose/high-cholesterol diet (HFSC diet) group; n=5 in the HFSC diet supplemented with NO-1886 (HFSC diet +NO-1886) group]. The total study period was 5 months. 2) The levels of glucose a