超声波辅助提取大豆油中豆甾醇工艺条件研究 study on the ultrasonic wave-assisted extraction of stigmasterol from soybean oil.pdfVIP

31 卷 4 期 皮 革 与 化 工 Vol. 31 No.4 2014 年8 月 LEATHER AND CHEMICALS Aug. 2014 论探讨 超声波辅助提取大豆油中 豆甾醇工艺条件研究 1 2 赵红红 ，刘彩芬 （1.陕西理工学院化学与环境科学学院，陕西汉中723001； 2.陕西理工学院生物科学与工程学院，陕西汉中723001 ） 摘要：以乙酸乙酯为提取剂，以大豆油为原料，采用超声波辅助提取大豆油中的豆甾醇，在单因素实验的基础 4 上，通过正交试验L (3 )对提取工艺进行了优化，结果表明，超声辅助提取大豆油中豆甾醇最佳工艺条件为：超 9 声温度50 ℃、超声时间40min 和液料比19mL/g 。在此最优条件下，豆甾醇的提取率最高，可达到34.15% 。 关键词：豆甾醇；大豆油；超声波辅助；提取 中图分类号：Q946.48 ；TQ420.6 文献标识码：A 文章编号：1674-0939（2014 ）04-0006-05 Study on the Ultrasonic Wave-assisted Extraction of Stigmasterol from Soybean Oil 1 2 ZHAO Hong-hong ，LIU Cai-fen (1. College of Chemistry Env ironmental Scie nce , Shaanxi Unive rs ity of Tec hnology, Hanzong 723001 ，China；2. College of Biological Scie nce Engineering, Shaanxi Unive rs ity of Tec hnology, Hanz hong 723001 ，China) Abstract: Selecting ethyl acetate as the extraction solvent, using soybean oil as raw material, the ultrasonic wave-assisted extraction conditions of stigmasterol from soybean oil were optimized through L 4 9 (3 ) orthogonal experiments on the base of single factor. The results showed that the optimum conditions were as follows: ultrasonic temperature 50 ℃, ultrasonic time 40 min, ratio of liquid to material 19 mL/g, and under these conditions, the extraction rate can reach to 34.15%. Key words: stigmasterol; soybean oil; ultrasonic