新型合成材料人工韧带的研制和动物实验研究.docVIP

何国础 刘 宏 杨庆铭 钱不凡 摘要 本实验用人工韧带是以涤纶为主要成分的合成材料 ,开放性编织成网孔状内 、外双套管结构 ;对 12 只狗的 16 个膝关节行前交叉韧带替代术 。阶梯状骨隧道生物学固定是其手术特征 。术后分期取材检 查 。18 周形态学显示新韧带已初具正常前交叉韧带形态 ,近骨隧道口韧带表面有微血管网形成 。组织学显 示新韧带表面滑膜组织内可见小血管长入 ,附着在人工纤维丝表面的胶原组织比例含量增高 ,排列致密 ,内 芯中央部分涤纶丝周边有丰富的按纵轴方向排列的胶原纤维附着 。电镜显示外套管人工纤维丝周边平行 排列的胶原纤维结构紧密 ,胶原纤维交联丰富 ,人工纤维丝间隙疏松组织比例下降 。力学测定显示人工韧带 18 周的极限承载能力为 473. 50 ±56 N , 与正常狗 ACL 比较差异无显著性意义 。短期实验表明 : 本韧带具有 较好的组织相容性和组织诱导性 ,并能承担相应的负荷 ,有一定的临床应用潜力 。 关键词 新型合成材料 人工韧带 组织诱导 力学性质 Development and Experimental Study of Artif icial L iga ment Ma de of A Ne w Type of Synthetic Material He Guochu , Li u Hong , Yang Qi ngmi ng , et al . Depa rtment of Orthopaedics , RuiJ i n Hospital affiliated to Shanghai Sec2 ond Medical Unive rsity , Shanghai Institute of Traumatology Orthopaedics , Shanghai 200025 Abstract The artificial ligament , which were consi sted of meshed i nner and outer doudle2tube structure and we re mai nl y weave d by pol ye ste r fi bre s , we re applie d to 16 knee s of 12 adult do gs i n the p roce dure of ACL reco n2 structio n i n thi s se rie s of e xpe ri me ntal study. The c haracte ri stic of thi s e xpe ri me ntal ope ratio n was biop hysical fi xa2 tio n by mea ns of steppi ng bo ne tunnel s. Biop sie s of reco nstructe d ACL we re obtai ne d at diffe re nt postope rati ve stage s. The reco nstructe d artificial li ga me nts we re with nearl y the sa me p re se ntatio n as normal ACL i n morp holo2 gy. The i ngrown small ve ssel s o n the surface of artificial li ga me nt , whic h forme d the mic ro2ci rc ulati ng retic ula de mo nstrate d by i rri gatio n of carbo n2blac k Chi ne se i nk i nto the fe moral arte ry , we re found locati ng o n the seg2 me nts nearby the bo ne tunnel s a nd al so we re co nfi rme d hi stolo gicall y i n the supe rficial synovial ti ssue . Unde r the obse rvatio n of electro n mic roscope , the p roportio n of i nte nsi ve arra nge d collage nous ti ssue whic h attac he d to the polye ste r fi bre s i nc rease d a nd o n th