牦牛气管中硫酸软骨素提取工艺研究.pdfVIP

1. Qinghai Academy of Agriculture and Forestry (Xining 810016) ; 2. Qinghai Province Tibetan Plateau Laboratory of Agric-product Processing (Xining 810016) Abstract Use ultrasonic wave to extract chondroitin sulfate from yak trachea, research on the effect of solid-liquid ratio, ultrasonic power and ultrasonic treatment time on chondroitin sulfate the yield. The technological optimum parameters were found as follows: solid-liquid ratio was 16 (g/mL), ultrasonic power was 550 W, ultrasonic treatment time was 30 min, and extraction temperature was 35 , pH 12, under these conditions, chondroitin sulfate the yield could reach 18.97%. The primary and secondary factors which affected the yield showed as following: ultrasonic powerultrasonic treatment time solid-liquid ratio. Three deproteinization methods: trichloroacetic acid method, Sevage method and parenzyme method were compared. It showed that the parenzyme method was the best, its protein removal rate could reach 85.78%, and purity of chondroitin sulfate was 85.56%. Keywords yak; chondroitin sulfate; ultrasonic wave; extraction