epsteinbarr病毒特异性细胞毒性t淋巴细胞在异基因造血干细胞移植中作用word格式论文.docx

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2018-05-18 发布于上海
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epsteinbarr病毒特异性细胞毒性t淋巴细胞在异基因造血干细胞移植中作用word格式论文.docx

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epsteinbarr病毒特异性细胞毒性t淋巴细胞在异基因造血干细胞移植中作用word格式论文

TheeffectofEpstein-Barrvirus-specificcytotoxicT lymphocytes inallogeneichematopoieticstemcell transplantationAbstractPart1:ExvivocultruedEpstein-Barrvirus specificcytotoxicT lymphocytes and evaluationofthekillingeffectObjectiveToexplorethemethodforinductionandexpandsionofEBvirusspecific cytotoxicTlymphocytes(EBV-CTL)invitro,anddetect thekillingeffect.Methods Peripheralbloodmononuclearcells(PBMNC)werecollectedfromsixEBVseropositive healthydonors,andEBV-transformedBlymphoblastoidcells(BLCL)wereusedastheantigen-presenting cells and antigen stimuliwhichwas irradiated by40Gy60Co. TheautologousPBMNCsandirradiatedBLCLswereculturedtoinduceandexpandthe EBV-CTLs,andtheimmunophenotypewasidentifiedbytheflowcytometry.Thekilling effectoftheEBV-CTLsagainsttheautologousBLCLs(autoBLCL),theautologousPHA culturedBlymphoblastoidcells(PHA-BLCL),theallogeneicBLCL(alloBLCL)andthe K562cellstrainweremeasuredwithLDHreleaseassayunderdifferenteffector-to-target ratio.ResultsSixcelllinesofEBV-CTLswereinducedandexpandedfromtheEBV seropositivehealthydonors,theoverallincreaseincellnumbersvariedfrom18.6to55.0 times.After10timesstimulations,thespecifickillingefficiencyoftheEBV-CTLsforthe autoBLCLwere59.4%,43.2%and29.0%undertheeffector-to-targetratioof20:1,10:1 and5:1.ThenonspecifickillingefficiencyforthePHA-Blast,alloBLCLandK562cellswere7.1%,9.4%and10.3%(p﹤0.05)underthe20:1ratio;6.6%,8.3%and8.1%(p﹤0.05)under10:1;5.4%,7.3%and6.3%(p﹤0.05)under5:1,respectively.Conclusion EBV-CTLcanbesuccessfullyinducedandexpandedexvivoforspecifickillingofHLA matched BLCLs, as could be the potentialtreatment for EBV related post-transplant英文摘要Epstein-Barr病毒特异性细胞毒性T淋巴细胞在异基因造血干细胞移植中的作用lymphoproliferativedisorders.Part2:Clinicalanalysis oflymphoproliferativedisorders afterallogeneic hematopoirticstem celltransplantationObjective Tostudytheearlydiagnosisofpost-transplantationlymphoproliferative disorders(PTLD)afterallogeneichematopoieticstemcelltransplantation(allo-HSCT)and investigatetheeffectivetherapystrategy.MethodsFrom