ercc1 ercc2 erbb1 erbb2 p53在膀胱尿路 上皮癌中的表达及意义word格式论文.docxVIP

目录中文摘要·······························································································1英文摘要·······························································································4研究论文ERCC1、ERCC2、ERBB1、ERBB2、P53在膀胱尿路上皮癌中的表达及意义前言·······························································································9材料与方法···················································································12结果·······························································································16附图·······························································································19附表·······························································································25讨论·······························································································35结论·······························································································38参考文献························································································39综述修复基因与恶性肿瘤的相关研究进展······································41致谢········································································································55个人简历································································································56ERCC1、ERCC2、ERBB1、ERBB2、P53 在膀胱尿路上皮癌中的表达及意义摘要目的：膀胱癌是泌尿系统最常见的恶性肿瘤，是人类十大常见肿瘤之一。其中90%为膀胱尿路上皮癌（bladderurothelialcarcinoma，BUC），BUC生长缓慢但容易复发。膀胱癌的发病原因非常复杂，迄今不能明确。现在针对肿瘤分子水平的研究飞速发展，证实肿瘤的发生过程是一个多基因参与、逐渐演进的过程，与癌基因的激活、抑癌基因的失活、修复基因的突变密切相关。因此发现新的分子生物学标记物对膀胱癌的早期诊断、评估预后、预防复发及设定个体化治疗至关重要。核苷酸切除互补交叉基因1（excisionrepaircross-complementinggene1，ERCC1）与核苷酸切除互补交叉基因2（excisionrepaircross-complementinggene1，ERCC2）是两个重要的DNA 修复基因。ERCC1参与DNA损伤识别和DNA链的切割。目前发现ERCC1与胃癌、非小细胞肺癌、食管癌、结直肠癌、膀胱癌的发生发展有关。ERCC2是损伤部位DNA解链所必需的酶。目前发现ERCC2在非小细胞肺癌中有异常表达。但关于ERCC1、ERCC2在BUC发生演进过程中的研究甚少。ERBB1、ERBB2是人类表皮生长因子受体（epidermalgrowthfactorreceptor EGFR）家族中两个重要的原癌基因。对于调节细胞的正常生长、增殖和分化有重要的作用，但体内ERBB1、ERBB2过表达将导致细胞异常增生，引发肿瘤。现已证实ERBB1在非小细胞肺癌、肾癌、结直肠癌中、乳腺癌、膀胱