急性冠脉综合症患者胸腺新近输出cd4 cd25 foxp3 调节性t细胞的检测及意义-detection and significance of cd4 cd25 foxp 3 regulatory t cells newly exported from thymus in patients with acute coronary syndrome.docx

下载文档 降价啦

4
0
约3.83万字
约 43页
2018-05-28 发布于上海
举报
版权申诉
保障服务

急性冠脉综合症患者胸腺新近输出cd4 cd25 foxp3 调节性t细胞的检测及意义-detection and significance of cd4 cd25 foxp 3 regulatory t cells newly exported from thymus in patients with acute coronary syndrome.docx

1、本文档共43页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

急性冠脉综合症患者胸腺新近输出cd4 cd25 foxp3 调节性t细胞的检测及意义-detection and significance of cd4 cd25 foxp 3 regulatory t cells newly exported from thymus in patients with acute coronary syndrome

TREC的数量为(894.17±415.127/106)，亦明显低于SA组 (1513.12±568.993/106, P=0.001)和HCs 组(1606.00± 497.720/106, P0.001)。而 SA组和HCs组间差异均无统计学意义 (P=0.106 和 P=0.566) 。患者 CD31+CD45RA+CD4+CD25+FOXP3+ 调节性 T 细胞比例与 CD4+CD25+CD127dim/-调节性T细胞中TREC的数量呈正相关(r=0.493, P=0.014)。实验结论急性冠状动脉综合征患者胸腺新近输出 CD4+CD25+FOXP3+调节性 T 细胞减少可能是外周血 CD4+CD25+FOXP3+调节性 T 细胞数量减少和功能受损的原因，亦可能是急性冠脉综合症的发生发展的原因。关键词急性冠脉综合症；CD4+CD25+FOXP3+调节性 T 细胞；胸腺新近输出；CD31；CD45RA；T 细胞删除环SignificanceoftheChangeofRecentThymic Emigrants CD4+ CD25+ FOXP3+ RegulatoryT Cells in Acute Coronary SyndromesPostgraduate Candidate: Ma Xuming Director: Li Da-zhuAbstractObjective: The numbers and suppressive function of CD4+CD25+FOXP3+ regulatory T cells (Treg) are impaired in patients with acute coronary syndromes (ACS). However, the mechanism underlying this phenomenon is unknown. Therefore, the aim of this study was to investigate whether recent thymic emigrants (RTE) Treg (RTE-Treg) contribute to the reduction of number and suppressive deficiency in Treg.Methods: 77 Subjects were divided into three groups: acute coronary syndromes (ACS, n=28), stable angina pectoris (SA, n=25) and healthy controls (HCs, n=24). Peripheral blood mononuclear cells (PBMC) were isolated by density gradientcentrifugation. We determined that express CD31+ combined CD45RA+ as recent thymicemigrant typical surface marker. The frequencies of CD31+CD45RA+CD4+CD25+FOXP3+ Regulatory T Cells (Treg) in peripheral blood were detected by flow cytometry. Meanwhile, we assess number of T-cell receptor excision circles (TREC) in Treg by real-time quantitative PCR to further investigate recent thymic emigrants CD25+FOXP3+Regulatory T Cells in patients with ACS.Results: Frequencies of CD31+CD45RA+ Treg are reduced in the peripheral blood of ACS patients (ACS vs SA, P=0.003 and ACS vs HCs P0.001). TREC numbers in Treg are reduced in the peripheral blood of ACS patients (ACS vs SA, P=0.001 and ACS vs HCs,P0.001). Meanwhile, frequencies of CD31+CD45RA+ Treg and TREC numbers in