荔枝核提取物对d-effect of litchi seed extract on d.docxVIP

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荔枝核提取物对d-effect of litchi seed extract on d.docx

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荔枝核提取物对d-effect of litchi seed extract on d

AbstractObjective:ToinvestigatetheeffectsofLitchiSeedExtract(LitchiSeedAqueousExtracts,LSAE)onlearningandmemorydisorderedinmurineinducedbyD-galactose,andstudythepossiblemechanismofaction.Method:2month-old50Kunmingmice,20±2g,sharingequalnumbersingenders,randomlydividedintonormalcontrolgroup(Normal,10mice)andmodelgroup(40mice).MiceinthemodelgroupwereinjectedwithD-galactose(500mg?kg-1?d-1)continuouslysubcutaneouslyfor8weeksinordertoestablishlearningandmemorydeficitsmodelwhilenormalcounterpartsweresubcutaneouslyinjectedwithsaline.Fromthe5thweek,the40micewerefurtherrandomizedintothefollowingsubgroupsandfedwithdrugsrespectivelyfor4weeksi.e.modelgroup(Model,10mice),huperzineAgroup(huperzine,A,HU,10mice,0.4mg?kg-1?d-1),litchiextracthighdosegroup(LSAEH,10mice,60g?kg-1?d-1)andlitchilowdosegroup(LSAEL,10mice,15g?kg-1?d-1),normalcontrolgroupandmodelcontrolgroupwereadministratedwithdistilledwater(10ml?kg-1?d-1).Fromthe8thweek,spontaneousactivityofmicewastestedbySMARTvideo-trackingsystemwhilethelearningandmemoryabilitieswereevaluatedbyMorriswatermaze.Usechemicalcolorimetrytomeasuresuperoxidedismutase(SOD)activity,andglutathioneperoxidase(GSH-Px)activity,malondialdehyde(MDA),advancedglycationendproducts(AGEs)contentinserum,andmeasurenitricoxide(NO)content,nitricoxidesynthase(NOS)activity,asacetylcholine(Ach)content,acetylcholinesterase(AchE)activityinthebraintissue.Flowcytometry(flowcytometer,FCM)wasadoptedtodeterminthepotentialofbraincellsmitochondrialmembrane.AGEsandAβofhippocampaltissuesweredetectedbyimmunohistochemistry.RT-PCRmethodwasusedtomeasurebcl-2mRNA,baxmRNAandβ-amyloidprecursorprotein(β-APP)mRNAexpressioninhippocampus.Transmissionelectronmicroscopywasusedtoobservethechangesofmitochondriastructure.Result:Comparedwiththenormalgroup,thespontaneousactivitiesinthemodlegroupweredecreasedsignificantly,withsloweraveragespeed(p0.05,p0.01)duringdayandnight,andincreasedrestingtimerates(p0.01,p0.01)duringdayandnight,shortenfastmotiontimerates(p0.01,p0.01)duringdayandnight,andincre