il-10启动子-1082和-819位点多态性与肠易激综合症的关系-the relationship between il - 10 promoter - 1082 and - 819 polymorphism and irritable bowel syndrome.docxVIP

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il-10启动子-1082和-819位点多态性与肠易激综合症的关系-the relationship between il - 10 promoter - 1082 and - 819 polymorphism and irritable bowel syndrome.docx

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il-10启动子-1082和-819位点多态性与肠易激综合症的关系-the relationship between il - 10 promoter - 1082 and - 819 polymorphism and irritable bowel syndrome

RoleofInterleukin-10GenePolymorphisminIrritableBowelSyndromeBackgroundName：JiChanglinSupervisor:NieYu-qiangABSTRACTIrritableBowelSyndrome(IBS)isacommonfunctionalboweldisorderaffectingasubstantialpropertionofthepopulation;pointprevalenceestimatesusuallyrangefrom5%-30%.Prevalenceratesvarysignificantlybetweencountries.TheetiologyofIBSiscomplexandappearstobemultifactorial,involvingalteredintestinalmotility,increasedvisceralsensitivity,psychosocialfactors,mucosalinflammation,dieryintoleranceandsoon.RecentlymanyresearchfoundIrritablebowelsyndromepatientsaggregatesinfamilies,perhapsthisisduetogeneticfactors.Someinvestigatorshaveproposedchroniclow-grademucosalinflammationasapotentialetiologicalfactorofIBS.Interleukin10(IL-10)isoneofthemostimportantanti-inflammatorycytokine，thereexistgenepolymorphysminthepromoterregionoftheIL-10.Astheelaborationofcytokinesisundergeneticcontrol，theremightbeageneticpredispositiontoanalteredpatternofanti-inflammatorycytokineproductioninpatientswithirritablebowelsyndrome.ThisstudywasdesignedtoestablishwhethertheremightbeageneticpredispositiontoanalteredpatternofIL-10productioninpatientswithirritablebowelsyndromeofguangdongpopulation.Objectives:Toinvestigatethegenepolymorphism-1082G/Aand-819C/TintheIL-10promoterregionandtheirrelationshiptoIBSinChinesepatients.Methods:ThreehundredandthirteenpatientswithIBSdiagnosedaccordingtoRomeⅢcriteriaand321healthycontrolswereenrolledtothisstudy.Bloodsamplesweretakenfromthepatientsandcontrols,thengenomeDNAwasextractedfromtheblood,andthenIL-10geneDNAwasamplifieddbypolymerasechainreaction(PCR),TheirIL-10-1082andIL-10-819genotypesweredetectedbyPCR-restrictionfragmentlengthpolymorphismanalysis.Results:TherewerenosignificantdifferencesinIL-10-1082A/Gand-819C/TallelefrequencydistributionbetweenIBSandcontrolgroup.Thefrequencyof-819T/TgenotypeinIBSgroup(51.1%）wassignificantmorefoundthancontrol(40.2%),whichwasalsomoreseenfrompatientsinthreeIBSsubgroups.TheIL-10-819TallelefrequencyinbothIBSwithdiarrhea(79.8%)andmixedIBSs