脑胶质瘤中ednrb基因甲基化状态的分析-analysis of methylation status of edn rb gene in glioma.docxVIP

脑胶质瘤中 EDNRB 基因甲基化状态的研究中文摘要目的抑癌基因启动子区异常甲基化是表观遗传学重要机制之一，参与了脑胶质瘤 的发生发展。本研究采用巢氏 PCR(nMSP 法)和亚硫酸氢盐修饰后测序法(BSP 法) 方法检测手术切除脑胶质瘤组织中候选抑癌基因 EDNRB 基因启动子区甲基化状 态，探讨 EDNRB 基因启动子的异常甲基化水平在脑胶质瘤发生发展中的作用以及 与临床病病理资料之间的关系。方法选取经病理证实的 20 例脑胶质瘤患者，术前均未行放疗和化疗，收集切除的 肿瘤组织。同时收集 2 例脑挫裂伤患者正常脑组织进行对照，应用 nPCR 方法和 BSP 方法检测标本中 EDNRB 基因启动子区 CpG 岛的甲基化状态。结果1.EDNRB 基因启动子区 CpG 岛的异常甲基化正常脑组织未见甲基化，低级别(I-II 级)胶质瘤中 EDNRB 基因启动子甲基化 率为 42.86％(3/7)，而高级别组(Ⅲ-IV 级) EDNRB 基因启动子甲基化率为 33.33％ (3/9)，通过分析表明两者之间无明显差别(X2=0.152.402, P=0.696)，患者年龄、性 别、肿瘤病理类型、肿瘤大小和肿瘤部位等因素与 EDNRB 基因甲基化无明显相关。结论EDNRB 基因的甲基化是肿瘤发生的早期事件，EDNRB 基因甲基化在脑胶质 瘤中较为普遍，与肿瘤进展基本无关。本研究没有发现 EDNRB 基因甲基化与患者 的年龄、性别、肿瘤病理类型、肿瘤大小等因素相关。关键词：内皮素受体 B 基因；启动子；甲基化；脑胶质瘤Methylation of EDNRB gene in gliomaAbstractObject：Methylation of Tumor-suppressor gene promoter is one of the important epigenetics mechanisms, participating in the glioma events. This study adopts nest PCR (nPCR) and hydrogen sulfite salt after modification sequencing method (BSP method)detecting candidated tumor-suppressor genes EDNRB gene promoter methylation in surgical resection of gliomas and area this group of patients postoperative patients receied follow-up survival, discussing the abnormal EDNRB gene promoter methylation gliomas occurrence and development, the relationship between role and clinical disease material.Methods：Selected the 20 patients with glioma patients were not line, preoperative chemotherapy and radiation, collect resection of the tumor tissue confirmed by pathology of. Meanwhile collecting 2 cases of normal brain tissue were compared, theapplication nPCR methods and ways to test the specimens BSP EDNRB gene promoter methylation CpG island area state.The results：EDNRB gene promoter area of abnormal methylation CpG island Low level (I - level II) in gliomas EDNRB gene promoter methylation rate to 21.43% (3/14), and the high level group (Ⅲ - level IV) EDNRB gene promoter methylation rate is 53.85% (7/13), are statistically significant differences between (X2 = 4.402), P = 0.037,shows that E