啤酒花浸膏的提取 浓缩及储藏性分析-extraction, concentration and storage analysis of hop extract.docxVIP

啤酒花浸膏的提取、浓缩及储藏性研究摘要啤酒花(Humunus lupulus L.)是桑科葎草属多年生草质蔓生藤本植物。啤酒花主要用 于啤酒酿造工业，赋予啤酒苦味和香味，同时亦作为一种药用植物被广泛使用。本研究 主要着眼于新鲜啤酒花的干燥，啤酒花浸膏的提取、浓缩及储藏性的研究。得出的结果 归纳如下：以新鲜啤酒花为原料，采用晒干、晾干、恒温鼓风干燥、常温鼓风干燥、冷冻干燥， 以啤酒花 a-酸，?-酸，总黄酮，黄腐酚含量为指标，研究了干燥方法对酒花品质的影响， 结果表明，常温鼓风干燥效果最好，a-酸含量为 7.34%、?-酸含量为 6.27%、总黄酮含 量为 69.10mg/g、黄腐酚含量为 1.24mg/g。采用微波辅助提取、超声波辅助提取、多级浸提法提取、常温浸渍法提取啤酒花浸 膏，其中以微波辅助法提取效果最好，通过响应面优化得到微波法提取啤酒花浸膏的最 优条件是：乙醇浓度为 58.8%、料液比为 1:20.6、提取时间为 58.5s，啤酒花浸膏提取液 的 a-酸含量为 5.318％。在单因素的基础上，通过正交试验确定了啤酒花浸膏浓缩的最优条件：真空度 0.06Mpa、水浴温度 55℃、浓缩时间 40min，该提取条件下真空浓缩啤酒花浸膏 a-酸得 率为 70.9%。啤酒花浸膏的品质分析：水分含量：=5%；密度：1.45g/mL-1.53g/mL；颜色：深粽 色；a-酸含量：20.00-22.72%；?-酸含量：17.00-19.20%；总黄酮含量：210.00-222.80mg/g； 黄腐酚含量：3.00-3.34mg/g。比较 8 种储藏方法，储藏期为 180 天，以啤酒花浸膏中 a-酸、?-酸、总黄酮、黄腐 酚含量为指标，得到-20℃真空储藏啤酒花有效成分损失最少。关键词：啤酒花；啤酒花浸膏；干燥；提取；浓缩；储藏Study on the Extraction,Concentration and Preservation of LupulinAbstractThe hop Plant (Humunus lupulus L.) is a dioecious twining Perennial. The inflorescences are used in the breweries to give beer its characteristics such as flavor and aroma and also as herbs. This paper aimed at arescent ing the fresh hop, research on the extraction, concentration and preservation methods of Lupulin. The main results as follows:Through taking fresh hop as raw material and a-acid, ?-acid, totle anthoxanthin and xanthohumol as the index, adopting open-air drying in the sun, open-air drying, blasting drying and lyophillization to dry fresh hop, the influence of drying methods on the quality of hop is studied . The results indicate: ordinary temperature blasting drying is the best one of which used to arescenting the fresh hop, and the content of a-acid is 7.34%, ?-acid is 6.27%, totle anthoxanthin is 69.10mg/g, xanthohumol is 1.24mg/g.Through adopting microwave method, supersonic wave method, many-stage lixiviation method and dipping method to extract Lupulin,the results indicate: microwave method is the best. Using response surface to analysis the optimization of condition: ethanol concentration is 58.8%, material: solvent