IL-1β及其Ⅰ型受体在大鼠肺纤维化发生过程中动态表达.docVIP

IL-1β及其Ⅰ型受体在大鼠肺纤维化发生过程中动态表达 　　[摘要] 目的:观察大鼠肺纤维化发生中IL-1β及其Ⅰ型受体(IL-1RⅠ)的动态表达。方法:采用气管内注入博莱霉素制备肺纤维化模型,设正常组、模型组和甲强龙组,于第1、3、7、14、28天分批处死大鼠,观察各组大鼠各期病理切片,检测肺泡灌洗液及血清中IL-1β和其Ⅰ型受体的含量。结果:模型组早期肺泡炎症明显,后期肺组织实变。灌洗液及血清中IL-1β于7 d时达峰值,14 d时显著下降,28 d时回到正常水平,Ⅰ型受体的表达规律与IL-1β相同。结论:IL-1β及其Ⅰ型受体在肺纤维化早期呈现短暂的表达高峰,提示其可能在肺纤维化发生的早期起关键作用。 　　[关键词] 肺纤维化;IL-1β;IL-1RⅠ 　　[中图分类号] R-332[文献标识码]A [文章编号]1673-7210(2010)03(c)-019-02 　　 　　Dynamic expression of IL-1β and IL-1RⅠ in the rats of pulmonary fibrosis 　　WANG Xiaohui, LIU Xuejun* 　　(Department of Geriatrics, the First Hospital of Shanxi Medical University, Taiyuan 030001, China) 　　[Abstract] Objective: To investigate the changes of IL-1β and IL-1RⅠ in pulmonary fibrosis induced by bleomycin in rats. Methods: The pulmonary fibrosis model was established by intratracheal injection of bleomycin with rats, set the normal group,the model group and the methylprednisolone group. The rats were killed on the 1st, 3rd, 7th, 14th and 28th day after treatment separately. The changes of biopsy of the lung tissue in every stage were investigated, the contents of IL-1β and IL-1RⅠ in BALF and blood serum were tested. Results: In the early period of pulmonary fibrosis, the inflammation in alveolar was obvious in the model group, while the lung tissue occurred consolidation at last. IL-1β in BALF and blood serum achieved peak on the 7th day, which was decreased markedly on the 14th day, and down to the normal level almost on the 28th day. While the expression pattern of IL-1RⅠ was similar to that of IL-1β. Conclusion: IL-1β and IL-1RⅠ in pulmonary fibrosis raise to the peak early and briefly, and play an important role in the earlier period of pulmonary fibrosis. 　　[Key words] Pulmonary fibrosis; IL-1β; IL-1RⅠ 　　 　　肺纤维化是多种不明原因的肺间质疾病的共同结局,近年来其发病呈增多趋势[1]。由于机制不清,至今仍缺乏治疗肺纤维化的有效手段,其病死率较高,预后较差[2],加强???其发病机制的研究,并在此基础上发展新的治疗策略已成为非常迫切的现实需要。目前国内外多数学者认为,炎症细胞及其释放的多种细胞因子在肺纤维化的发病机制中起中心环节的作用[3]。IL-1β是体内作用最强的前炎症细胞因子之一,广泛参与机体多种疾病的发生和发展[4]。有研究报道,在肺纤维化的动物模型和特发性肺间质纤维化患者的支气管肺泡灌洗液