OPCs移植对EAE大鼠脊髓有髓神经纤维髓鞘形态及MBP表达影响.docVIP

OPCs移植对EAE大鼠脊髓有髓神经纤维髓鞘形态及MBP表达影响 　　[摘要] 目的 观察少突胶质前体细胞（OPCs）移植对实验性变态反应性脑脊髓炎（EAE）大鼠脊髓有髓神经纤维髓鞘形态的影响。 方法 选择30只健康大鼠随机分为正常组、模型对照组、治疗组，每组10只。正常组正常饲养，模型对照组、治疗组采用大鼠脊髓匀浆与完全弗氏佐剂混合做抗原单点皮下注射造模，造模后1周治疗组由尾静脉注入OPCs悬液，模型对照组注入同等剂量生理盐水，连续测量大鼠体重的改变，将各组大鼠心脏灌注处死，取脊髓制成切片，用髓鞘碱性蛋白（MBP）免疫组化染色并观察。 结果 造模后1周模型对照组与治疗组大鼠体重均下降。移植后，治疗组大鼠体重下降有所缓解；与正常组比较，模型对照组大鼠运动功能评分降低，差异有统计学意义（P0.05）；与模型对照组比较，治疗组运动评分升高，差异有统计学意义（P0.05）；与模型对照组比较，治疗组MBP免疫组化阳性的髓鞘数量增多。 结论 OPCs移植可促进脱髓鞘模型大鼠运动功能和脊髓髓鞘的修复。 　　[关键词] 多发性硬化；少突胶质前体细胞；细胞移植；实验性变态反应性脑脊髓炎 　　[中图分类号] R651.21 [文献标识码] A [文章编号] 1674-4721（2015）05（b）-0004-03 　　Influence of oligodendrocyte precursor cells transplantation to the structure of myelinated nerve fibers in spinal cord and the expression of MBP 　　ZHANG Meng1，2 ZHANG Hai-yan2 YAO Hong-bo2 WANG Yue-jing2 SUN Li-hui2▲ 　　1.Human Anatomy，Histology and Embryology Teaching and Research Section，Heilongjiang University of Chinese Medicine，Heilongjiang Province，Harbin 150040，China； 2.Histology and Embryology Teaching and Research Section，Qiqihar Medical University，Heilongjiang Province，Qiqihar 161006，China 　　[Abstract] Objective To observe the influence of oligodendrocyte precursor cells transplantation to motor function and myelinated nerve fiber morphology of experimental allergic encephalomyelitis rats. Methods 30 healthy rats were randomly divided into normal group，model control group，treatment group.Normal group was given normal breeding，spinal cord homogenate and complete Freund′s adjuvant were mixed and injected into rats in the model control group and treatment group.About 1 week later，the treatment group rats were given intravenous injection of oligodendrocyte precursor cells，model control group was injected equal dose of normal saline，the change of weight in rats were measured continuously.Rats in each group were killed by cardiac perfusion，spinal cord was made into slices，MBP immunohistochemical staining was given and observed. Results Compared with the normal group，the weight of model control group decrea