TGF―β1抑制剂Decorin对子宫内膜癌细胞HBC―1B迁移侵袭转移能力影响.docVIP

TGF―β1抑制剂Decorin对子宫内膜癌细胞HBC―1B迁移侵袭转移能力影响 　　[摘要]目的 研究?D化生长因子-β1（TGF-β1）及其抑制剂Decorin对子宫内膜癌细胞迁移、侵袭转移能力的影响。方法 将子宫内膜癌细胞HEC-1B进行体外培养，实验设阴性对照组（未经TGF-β1及Decorin处理）、TGF-β1组（TGF-β1的处理浓度为10 ng/ml）、Decorin 组（Decorin的处理浓度为100 ng/ml）、TGF-β1及Decorin组（TGF-β1的处理浓度为10 ng/ml，Decorin的处理浓度为100 ng/ml），TGF-β1及Decorin分别作用于人子宫内膜癌细胞HEC-1B后，MTT法检测其对子宫内膜癌细胞的增殖抑制作用。Transwell实验检测TGF-β1及Decorin对子宫内膜癌细胞迁移、侵袭转移能力的影响。结果 Decorin明显抑制子宫内膜癌HEC-1B细胞的体外增殖，与阴性对照组比较差异有统计学意义（P0.05） ，而TGF-β1及Decorin组HEC-1B细胞的迁移数和侵袭数较TGF-β1组明显减少（P0.05）。结论 体外TGF-β1能够增强子宫内膜癌细胞HEC-1B的迁移、侵袭转移能力，而TGF-β1抑制剂Decorin可抑制子宫内膜癌细胞的增殖和侵袭转移。 　　[关键词]子宫内膜癌；侵袭转移能力；TGF-β1；核心蛋白聚糖 　　[中图分类号] R737.33 [文献标识码] A [文章编号] 1674-4721（2017）09（b）-0004-04 　　The influence of TGF-β1 inhibitor-Decorin on the invasion and migration ability of HEC-1B for endometrial cancer cell 　　CAI Yu DIAO Hai-dan 　　Department of Gynecology，the Third People′s Hospital of Dalian City in Liaoning Province，Dalian 116033，China 　　[Abstract] Objective To study the influence of TGF-β1 inhibitor Decorin on the invasion and migration ability of HEC-1B for endometrial cancer cell.Methods Endometrial cancer cell HEC-1B was cultured in vitro.Negative control group （without TGF-β1 and Decorin treatment） ，TGF-β1 group （10 ng/ml of treatment concentration for TGF-β1） and Decorin group （100 ng/ml of treatment concentration for Decorin），TGF-β1 and Decorin group （treatment concentration of TGF-β1 was 10 ng/ml，Decorin treatment concentration of Decorin was 100 ng/ml） were established.After TGF-β1 and Decorin actting on endometrial cancer cell HEC-1B respectively，MTT method was used to detect the proliferation and inhibition role of TGF-β1 and Decorin on endometrial cancer cell HEC-1B.The influence of TGF-β1 and Decorin on the invasion and migration ability of HEC-1B for endometrial cancer cell was examined by Transwell experimention.Results Decorin inhibited obviously the proliferation in vitro endometrial cancer cell HEC-1B and there was a statistical difference compared with negative con