TGFβ1与Smad4在大肠癌组织中表达及意义.docVIP

TGFβ1与Smad4在大肠癌组织中表达及意义 　　[摘要] 目的：探讨TGF-β1与Smad4在大肠癌组织中的表达及其与大肠癌发生发展的关系。方法：应用免疫组织化学SABC法检测67例大肠癌组织中TGF-β1与Smad4蛋白表达。并选择24例正常人大肠黏膜组织作为正常对照组。结果：大肠癌组织中TGF-β1蛋白的阳性表达率为67.2%，较正常对照组(4.2%)明显增高(P0.05)；大肠癌组织中Smad4蛋白的阳性表达率为44.8%，较正常对照组(95.8%)明显(P0.05)降低；TGF-β1和Smad4在大肠癌组织中的表达呈负相关(r=-0.521；P＜0.01)。结论：高表达的TGF-β1和低保留表达的Smad4在大肠癌发生发展中起重要作用，且二者作用具有相关性。 　　[关键词] TGF-β1；Smad4；大肠癌 　　[中图分类号] R735.3+4[文献标识码]A [文章编号]1673-7210（2008）09（c）-007-02 　　 　　The expression and biological significance of TGF-β1 and Smad4 in colon carcinoma tissue 　　LI Shi-kun, CHEN Ke-he, REN Qing-mei 　　(Department of Gastroenterology, Xinyang Central Hospital of Henan Province, Xinyang 464000, China) 　　[Abstract] Objective: To explore the significances of TGF-β1 and Smad4 expressed in the progression aggressiveness of colon carcinoma. Methods: The protein expression of TGF-β1 and Smad4 were detected in specimens from 67 cases of colon carcinoma and 30 cases of normal colon mucosa tissues with immunohistochemical method. Results: Our results showed that the positive rate of TGF-β1 in colon carcinoma tissue was significantly higher than that in normal colon tissue(67.2% vs 4.2%; P0.05), while the positive rate of Smad4 protein expression was lower than that in normal colon tissue(4.8% vs 95.8%; P0.05). The negative correlation was observed between the positive expression of TGF-β1 and preserved expression of Smad4(r=-0.521; P＜0.01). Conclusion: The positive expression of TGF-β1 and reduced expression of Smad4 may be closely related to the genesis and development of colon carcinoma. There is a negative correlation between the expression of TGF-β1 and that of Smad4. 　　[Key words] TGF-β1; Smad4; Colon carcinoma 　　 　　TGF-β1(tansforming growth β1)在各种肿瘤中的过度表达与临床病理特征及肿瘤患者预后的密切关系，中外文献已有报道[1,2]，而其与Smad4在大肠癌中的作用机制比较复杂[3-5]。本实验采用免疫组织化学方法联合检测二者在大肠癌组织中的表达情况及相互关系，旨在探讨它们在大肠癌发生发展中及癌细胞逃逸TGF-β1负调控的可能机制，为临床上从TGF-β1角度探讨抑制肿瘤发生发展的新途径提供理论依据。 　　1 材料与方法 　　1.1 研究对象 　　大肠癌组67例，均系我院病理科部分大肠癌手术切除组织的石蜡标本，其中，男性45例，女性22例，年龄29～75岁，平均52