不同血液标本处理方案下生化检查结果差异对比.docVIP

不同血液标本处理方案下生化检查结果的差异对比 　　【摘要】 目的：探讨对血液标本进行不同处理后产生的生化结果的影响。方法：将笔者所在医院90例进行血液检验的患者随机分为1组、2组、3组和A组、B组、C组，另选30名身体各项指标均健康的志愿者为参考组，进行不同处理后比较1、2、3组的生化结果与参考值差异，1组4 ℃保存24 h后离心，2组取样20 min后离心，4 ℃保存、3组20 min内分离血清检测；将A组设为对照组将其静置20 min后分离血清进行检测，参考组进行正常的血液标本处理并检测，对B组、C组进行全血保存和血清分离保存，之后检测两组得出结果。结果：3组的处理方式对检测结果无影响（P0.05），1组、2组对生化检测结果有明显影响（P0.05），1组影响最明显，相比较C组的血清分离保存，B组的全血保存对GLU及LDH指标有明显影响。结论：对血液标本的处理方式不同会引起生化指标的差异，故不能及时处理的标本应将其离心冷藏，以保证得到正确的生化指标，确保疾病诊断的准确性。 　　【关键词】 血液标本； 不同处理方法； 生化检查 　　中图分类号 R446.1 文献标识码 B 文章编号 1674-6805（2015）30-0047-03 　　【Abstract】 Objective： To investigate the effect of biochemical results after different treatment on blood samples.Method： 90 cases of hospital blood tests were randomly divided into group 1 group，2 group，3 group and A group，B group，C group，select the body of the indicators were healthy volunteers as the reference group，different treatments after 1 group，2 group，3 group of biochemical results with the reference value of the difference，1 group of 4 ℃ 24 h after centrifugation，two groups of sampling for 20 minutes and centrifuged，4 ℃，3 group within 20 min to separate serum detection.The A group as control group the static 20 min after separation of serum were detected，the reference group of normal blood samples and detection of B group and C group of whole blood and serum separation and detection of two groups of results.Result：The 3 group had no effect on the test results（P0.05），1 group and 2 group had obvious influence on the biochemical test results（P0.05），the effect of the 1 group was the most obvious，and the whole blood of B group was significantly affected by GLU and LDH.Conclusion：The treatment method of blood samples will cause the difference of biochemical parameters，so the samples should be treated by centrifugal refrigeration，so as to ensure the accuracy of disease diagnosis. 　　【Key words】 Blood specimens； Different treatment methods； Biochemical tests 　　First-author’s address：Guangnan People’s Hospital，Guangnan 663300，China 　　doi：10