VEGF在大鼠实验性脑积水脑组织中的表达-外科学专业论文.docxVIP

中文摘要 目的建立成年大鼠脑积水模型，探讨血管内皮叶飞长因子 (VEGF) 在脑组织中的表 达状况。 方法雄性 Wistar 大鼠 60 只，随机分为实验组和对照组，其中实验组 48 只，再 随机平均分为 A 、8 ，C ，D 囚个亚组 @应用显微外科技术向枕 大池内注入 25% 白陶土 混悬液 0.1m!. 分别在白陶土注射后第 1、2 、4 、6 周行 MRI 检测，获取鼠脑冠状切 面的飞图象，鉴定脑积水是否形成。之后 24 小时内处死动物并迅速取出脑组织，应 用免疫组织化学方法分别测定各组大鼠脑组织不同部位 VEGF 表达情况。其余 12 只 用同样方法向枕 大池内注入牛理然水作为对 照、级 。 结果实验组 34 只大鼠成功诱发脑积水，脑室系统的扩张程度随着时间的延长 运渐明显。脑积水形成后，脑组织中 VEGF 表达明显升高，尤其 是脑室周围白质和海 马更为 明显。 结论 枕大池白陶土注入法可以制作确切的大鼠脑积水模型，适用于急、慢性脑 积水的实验研究。脑组织中 VEGF 表达升高，一方 面与脑室周围组织水肿有关， 另一 方面可能对脑积水导致的缺血性损害有神经保护作用。 VEGF 参与了脑积水的病理损 害及修复过程。 关键词血管内皮生长因子脑积水大鼠 臼陶土 Abstract Objective: To establish anímal model of hydrocephalus in adult rat，and study the expression of vascular endothelial growth tactor(VEGF) in the cerebral tissue of different sltes. Methods 60 adult male 轨listar rats were randomly divided into experiment group，where 4日 rats were randomly divided equally into four subgroups ，ie.. group A 、8、 C and D ， and co口trol group. The animal models of hydrocephalus were established by injecting O.1ml volume of25% kaolin solutíon ，有ith the aid of surgical microscope into the cisterna magna in the 48 rats in the experiment group. The s缸丑e volume of saline was 1均ected into the cisterna magna ín the r础。f the control group..MRI was performed l(group A) 、2(group B) 、4(group C) or 6(group D) weeks after Kaolin injection respectively to obtain T2-weighted images of brains of the rats. The rats were killed within 24 且oursa我erM阳 examination，and the braìns were removed out quìckly for studying the VEGF expressíon by the ímmunohistochemis町 method Results Hydrocephalus was induced in 34 rats，the ventricular system expanded along with the time passed by.The expression of VEGF is significantly íncreased in the cerebral tissue of rats with hydrocephalus.especially in the periventτicular white matter and hippocampus Conclusion: The techníque presented here for establishing anímal model of hydrocephalus in adult rat is reliable .lt is particularly suitable for study