TLR9参与调节高糖诱导心肌纤维化-心血管内科专业论文.docxVIP

Abst Abstract IV IV ABSTRACT Objectives: The purpose of this study is to explore the relationship between toll-like receptor 9（TLR9 ）and myocardial fibrosis induced by high glucose . Methods: We cultured cardiac fibroblasts with high glucose medium and observed the proliferation response and inflammation response of cardiac fibroblasts to prove that pretreatment with high glucose can induce proliferation and inflammation of the cardiac fibroblast. Then transfected cardiac fibroblasts with TLR9 low expression lentiviral, still cultured with high glucose medium and observed the changes of proliferation and inflammatory response in cardiac fibroblast, which used to certified that high glucose can eventually induced myocardial fibrosis by proliferation and inflammation of cardiac fibroblasts via regulate TLR9 expression levels. Cardiac fibroblasts were divided into the following six groups as our experimental design, normal control group: cardiac fibroblasts cultured with 5.5 mmol/L normal glucose medium for 48 hours; high glucose group: cardiac fibroblasts pretreatment with 25 mmol/L high glucose medium for 48 hours; high glucose+TLR9-RNAi-LV group: replace the cardiac fibroblasts medium with 25 mmol/L high glucose medium for 48 hours after the successful transfection of cardiac fibroblasts with TLR9 low expression lentiviral; normal glucose+TLR9-RNAi-LV group:cardiac fibroblasts cultured with 5.5 mmol/L normal glucose medium for 48 hours after the successful transfection of cardiac fibroblasts with TLR9 low expression lentiviral; high glucose+NC-LV group: replace the cardiac fibroblasts medium with 25 mmol/L high glucose medium for 48 hours after the successful transfected with empty virus; normal glucose+NC-LV group: cultured the cardiac fibroblasts with 5.5 mmol/L normal glucose medium for 48 hours after transfected with empty virus. After 48 hours, we detecte the expression levels of TLR9 ,inflammatory cytokines of IL-6, IL-1β and TNF-a, myocardial fibrosis f