TIF1γ可以抑制TGFβ诱导小鼠肝细胞发生EMT及增殖抑制作用-肝脏外科专业论文.docxVIP

原 创 性 声 明 本人郑重声明： 所呈交的学位论文，是本人在导师的指导下，遵照严肃求实的 科学精神，独立进行研究工作所取得的成果。论文中除已注明引用和致谢的内容外， 不包含其他个人或集体已经发表或撰写过的研究成果。对论文研究做出贡献的个人或 集体，均在论文中作了明确声明并表示了谢意。本声明的法律责任由本人承担。 论文作者亲笔签名： 日期 ： 学位论文版权使用授权书 本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保 留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本 人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索， 可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。 本论文属于 保密□， 在 年解密后适用本授权书。 不保密□。 （请在以上方框内打“√”） 学位论文作者签名： 指导教师签名： 日期： 年 月 日 日期： 年 月 日 华 中 科 技 大 学 硕 士 学 位 论 文 1 1 目 录 中英文缩写对照表 ················································ 摘要 ·································································2 Abstract 3 前 言 ······························································4 第一章 TIF1γ 敲减及过表达稳定细胞系的建立 ·········6 引 言···································································································· 6 材料与方法······························································································ 7 实验结果································································································ 13 本章讨论································································································ 13 第二章 TIF1γ 阻断 TGFβ 对小鼠肝细胞的作用 ········· 14 引 言···································································································14 材料与方法····························································································· 15 实验结果································································································ 22 本章讨论································································································ 27 全文小结 ························································· 28 综 述 ···························································· 29 致 谢 ···························································· 40 中英文缩写对照 英文缩写 英文全名 中文全名 TIF1γ Transcriptional intermediary factor-1γ 转录中介因子 1γ TGF-β Transforming Growth Factor-beta 转换生长因子-β EMT Epithelial-Mesenchymal Transition EMT 上皮间质转化 PBS phosphate buffered saline 磷酸盐缓冲液 BSA Bull Serum Alb