T型钙通道在膀胱起搏细胞(ICCs)兴奋调控中的作用及机制初探-外科学(泌尿外)专业论文.docx

第三军医大学博士学位论文 第三军医大学博士学位论文 PAGE PAGE 1 缩略语表 英文缩写 OAB 英文全称 overactive bladder 中文全称 过度活动膀胱 ICC interstitial cell of Cajal 卡哈尔间质细胞 PBOO partial bladder outlet obstruction 部分膀胱出口梗阻 WB western blotting 免疫印迹 CCH Carbachol 卡巴可 DI detrusor instability 逼尿肌不稳定 AP action potential 动作电位 BSA bovine serum albumin 牛血清白蛋白 SMC smooth muscle cell 平滑肌细胞 cAMP cyclic adenosine monophosphate 环磷酸腺苷 cGMP cyclic guanosine monophosphate 环磷酸鸟苷 Cm membrane capacitance 膜电容 DAPI 4,6-diamidino-2-phenylindole 4,6-二脒基-2-苯基吲哚 DEPC diethypyrocarbonate 焦碳酸二乙酯 DMSO dimethyl sulfoxide 二甲亚砜 FBS fetal bovine serum 胎牛血清 FITC Fluoresce inisothiocyanate 异硫氰酸盐荧光素 h hour 小时 LVA Low voltage activated-channels 低电压活化通道 HBSS Hank’s balanced salt solution Hank’s 平衡盐溶液 HEPES Hydroxyethyl piperazine ethanesul fonicacid 羟乙基哌嗪乙磺酸 TP test potential 检测电压 HP holding potential 钳制电位 IF immunofluorescence 免疫荧光染色 Kit receptor tyrosine kinase 酪氨酸激酶受体 M mol/L 摩尔每升 min minute 分钟 PBS phosphate buffered saline 磷酸盐缓冲液 RFI relative fluorescent intensity 相对荧光强度 rpm Rounds per minute 每分钟转速 SCF stem cell factor 干细胞因子 SD standard deviation 标准差 SMM smooth muscle myosin 平滑肌肌球蛋白 TP testing potential 测试电压 TRITC tetraethyl rhodamine isothiocyanate 四乙基罗丹明异硫氰酸盐 FITC fluorescein isothiocyanate 异硫氰酸荧光素 PVDF polyvinylidene fluoride 聚偏二氟乙烯 IC interstitial cell 间质细胞 The role and mechanism of T-type calcium channels in the bladder ICCs excitement regulation Abstract Background and purposes: Overactive bladder (OAB) is a class of the abnormal voiding dysfunction syndromes, with urgency as main complaint, with or without complaint to urge incontinence, often with frequent urination or nocturia. This disease can occur independently, but also is associated with a variety of urological diseases, such as urinary tract infections, benign prostatic hyperplasia, senile voiding dysfunction, neurogenic bladder, urinary tract obstruction, interstitial cystitis / painful bladder syndrome and lower urinary tract tumors, urinary stones, unexplained male and fema