VEGF在他汀类药物干预易损斑块形成的表达-药理学专业论文.docxVIP

摘 摘 要 I I 摘 要 目的： 本研究使用辛伐他汀对动脉粥样硬化动物模型实施早期干预，通过检测不同组兔血 清中 VEGF 水平变化，探讨辛伐他汀对 VEGF 表达的影响；通过观察干预与非干预情况 下早期粥样硬化斑块病理学改变及斑块纤维帽帽/核比值变化。从而探索 VEGF 在动脉 粥样硬化斑块形成发展过程中的作用，及其对于粥样斑块易损相关性的影响。 方法： 实验用新西兰大白兔 18 只。随机分为 A 组（正常对照组）6 只，B 组（模型组）6 只，C 组（辛伐他汀干预组）6 只。分别给予正常饲料及高脂饲料喂养 2 周后，腹主动 脉球囊拉伤法制作动脉硬化斑块模型组，喂养 6 周，三组动物行腹主动脉超声检查明确 动脉硬化斑块形成；B 组阴性对照，C 组给予辛伐他汀干预治疗，继续喂养 4 周后测定 各组 VEGF 血清水平。对腹主动脉粥样硬化斑块进行病理切片，大体染色，并对硬化斑 块纤维帽、脂核进行测量，计算帽核比值，评估斑块易损性，对 VEGF 及帽核比值相关 性进行分析。 结果： 1. 辛伐他汀干预组 VEGF 血清浓度较模型组明显下降。 2. 辛伐他汀干预组纤维帽/脂核横截面积比值较模型组明显上升。 3. 血清 VEGF 浓度与动脉粥样硬化斑块帽核比值上升呈负相关。 结论： 提示 VEGF 在影响硬化斑块易损性方面存在密切相关性。血清中 VEGF 浓度可作为 反应粥样硬化斑块易损性指标之一。 关键词 动脉粥样硬化斑块 易损性 血管内皮生长因子（VEGF） 辛伐他汀 帽核比值 Abstract Abstract II II Abstract Objective: The research is to observing vascular endothelial growth factor(VEGF) in blood serum after animal model of AS interfering by simvastatin, exploring the effect of simvastatin toward to the expression of VEGF. Evaluate the effect of VEGF toward to development process of atherosclerotic plaque and vulnerability of atherosclerotic plaque by observing early term pathology results of atherosclerotic plaque interfering by simvastatin or not. Methods: 18 New Zealand white rabbits were divided into three groups randomly, group A(normal control), group B(model of AS), group C(simvastatin intervention).Group A and B were fed a 2% cholesterol diet for two weeks. The rabbits’ right femoral artery was surgically exposed, a small arteriotomy site created, and a 4F Fogarty embolectomy catheter was advanced into the abdominal aorta up to the level of the diaphragm. The catheter was then inflated to a pressure and pulled to the bifurcation of the aorta. After 3 such passes, the surgical cutdown site was closed.The animals were continued on the hyperlipidemic diet for 12 more weeks and were made the atherosclerotic model. Group C were interfering by simvastatin. The rabbits were euthanized.The entire length of the aorta was exposed and was divided several segments.Then histological assessment of exper