异叶茴芹提取物抗炎止血凝血作用实验研究.docVIP

异叶茴芹提取物抗炎止血凝血作用实验研究 　　摘要：目的研究异叶茴芹提取物抗炎、止血、凝血作用功效，为异叶茴芹的潜在药用价值开发利用提供依据。方法选取健康小鼠，采用二甲苯致小鼠耳廓肿胀，测定异叶茴芹醇提液和水提液的抗炎作用效果；采用小鼠断尾法止血实验、小鼠载玻片法凝血实验评价异叶茴芹醇提液和水提液的止血和凝血作用效果。结果异叶茴芹提取物灌胃能减轻二甲苯致小鼠耳廓肿胀程度，水提液组其足肿胀抑制率达24.51%，缩短剪尾法所致小鼠出血时间，水提液组其缩短率为33.41%，加速实验小鼠的凝血时间，醇提液组其缩短率达47.59%。结论异叶茴芹提取物具有一定程度的抗炎、止血、凝血活性，具有综合利用前景。 　　关键词：异叶茴芹；抗炎；止血；凝血 　　中图分类号：R285.5文献标志码：A文章编号：1007-2349（2017）11-0075-03 　　【Abstract】Objective： To study the anti-inflammatory， hemostatic and coagulation effects of extracts from Pimpinella diversifolia and to provide the basis for the development and utilization of its potential medicinal value. Methods： The healthy mice were selected to induce their ear swelling by xylene to determine the anti-inflammatory effect of ethanol extract and aqueous extract of Pimpinella diversifolia. The tail-cut hemostasis experiment and slide glass coagulation experiment were used to evaluate the effect of hemostatic and coagulation effects of ethanol extract and aqueous extract of Pimpinella diversifolia. Results： The intragastric administration of Pimpinella diversifolia extracts could reduce the degree of auricle swelling in xylene-induced mice. The inhibition rate of foot swelling in the aqueous extract group was 24.51%. The tail-cut hemostasis shortened the bleeding time of the mice， and the shortened rate of the aqueous extract group was 33.41% and the coagulation time of the mice was accelerated. The shortened rate of alcohol extract was 47.59%. Conclusion： The extracts of Pimpinella diversifolia has certain anti-inflammatory， hemostatic and coagulation activity and has comprehensive utilization prospect. 　　【Key words】Pimpinella diversifolia， anti-inflammatory， hemostasis， blood coagulation 　　异叶茴芹（Pimpinella diversifolia DC.）为伞形科茴芹属植物，别名为八月白、苦爹菜、山当归、鹅脚板、蛇倒退。野生植物资源丰富，是民间常用草药和野菜之一。以全草入药，具有温中散寒、宣肺祛痰、活血止痛、解毒消肿等作用[1-6]。茴芹属植物中主要含有挥发油及萜类化合物、香豆素类化合物、黄酮类化合物、甾醇类化合物和脂肪酸类化合物等[7]，这些化合物的存在，可以对异叶茴芹的药用功效给出合理的解释。异叶茴芹虽在民间用药中历史已久，但是其药用价值的挖掘工作还很有限，其植物提取物的动物实验至今还鲜有报道。笔者针对异叶茴芹提取物抗炎、止血、凝血作用功效展开了研究，