常春卫矛提取物体外及体内抑菌作用试验研究.docVIP

常春卫矛提取物体外及体内抑菌作用试验研究 　　[摘要] 目的 研究常春卫矛水提物、醇提物及5种不同溶剂提取物的抑菌活性。 方法 体外抑菌试验采用试管二倍稀释法联合琼脂平板法，考察常春卫矛水提物、醇提物及5种不同溶剂（石油醚、氯仿、乙酸乙酯、正丁醇、甲醇）提取物对金黄色葡萄球菌、大肠埃希菌、沙门菌、铜绿假单胞菌的体外抑菌活性；体内抑菌试验以金黄色葡萄球菌为指示菌种，小鼠灌胃分别给予常春卫矛水提物、醇提物14 d，腹腔注射金黄色葡萄球菌，通过观察小鼠死亡率衡量常春卫矛提取物的体内抑菌活性。 结果 体外抑菌试验显示，常春卫矛水提物及醇提物均对金黄色葡萄球菌、大肠埃希菌、沙门菌、铜绿假单胞菌有一定程度的抑制作用，5种不同溶剂提取物中，甲醇、正丁醇、乙醇乙酯提取物对金黄色葡萄球菌、大肠埃希菌、沙门菌、铜绿假单胞菌有一定程度的抑制作用，石油醚、氯仿提取物无明显抑制作用。体内抑菌试验显示，常春卫矛水提物低（16.0 g/kg）、高（32.0 g/kg）剂量组、醇提物高剂量组（32.0 g/kg）有明显降低金黄色葡萄球菌感染小鼠死亡率的作用（P0.05）。 结论 常春卫矛提取物具有一定程度的抑菌作用。 　　[关键词] 常春卫矛；提取物；体内；体外；抑菌作用 　　[中图分类号] R282[文献标识码] A[文章编号] 1674-4721（2014）06（c）-0009-04 　　 　　Experimental study in vitro and in vivo antibacterial effect of the extract of Euonymus hederaceus 　　LUO Yu-dong ZHANG Qiang▲ JIANG Lin LI Na 　　Pharmaceutical Factory of Guangxi University of Chinese Medicine,Nanning 530023,China 　　[Abstract] Objective To investigate antibacterial activity of Euonymus hederaceus water extract,alcohol extract and 5 different solvent extracts. Methods In vitro inhibitory effect of Euonymus hederaceus water extract,alcohol extract and different solvent extracts(petroleum ether,chloroform,ethyl acetate,n-butanol,methanol)on Staphylococcus aureus,Escherichia coli,Salmonella and Pseudomonas aeruginosa were assayed by the method of tube double dilution method combined with agar plate method.In vivo inhibitory effect of the Euonymus hederaceus water and alcohol extraction with Staphylococcus aureus as index,the mice were given Euonymus hederaceus water and alcohol extraction by gavages for 14 days,then the mice were intraperitoneally injected with Staphylococcus aureus suspension.In vivo antibacterial activity of Euenymus hederaceus watder and alcohol extraction was detected respectively by observing the mortality rate in mice. Results Bacteriostatic test in vitro showed that Euonymus hederaceus water extract and alcohol extract on Staphylococcus aureus,Escherichia coli,Salmonella,Pseudomonas aeruginosa had certain degree of restrained.Different solv