不同海拔地区藏、汉族健康人脑血管反应性及血浆NO、eNOS含量的研究-内科学(神经病)专业论文.docxVIP

青海大学 青海大学 2010 级攻读硕士学位研究生毕业论文 第 第 3 页，共 32 页 Abstract Cerebrovascular Reactivity and NO, eNOS Plasma Level in Healthy Adults between Tibetan and the Han Chinese at Different Altitude Objective By detecting cerebrovascular reactivity, the plasma level of NO, eNOS among Tibetan , Han Chinese population at the same altitude, Han Chinese population from different altitude respectively , to discuss the characteristics of cerebrovascular reactivity and its underlying mechanisms and explore the effects on cerebrovascular regulation of plasma NO and plasma eNOS. Methods Selected 45 people from Qinghai Province, Guoluo Prefecture on elevation of 3800m by the physical examination to exclude high blood pressure, diabetes, high cholesterol and heart, cerebrovascular diseases and vascular conditions of Tibetan in young adults (ages 25 to 45 years old) as experimental group 1, selected 45 Han Chinese subjects who lived in high altitude more then 10 years as a experimental group 2, as well as selected 45 Han Chinese subjects who lived in moderate altitude in Xining as a experimental group ; In addition selected low altitude Han Chinese subjects from SiChuan Province, Guanghan (450m) as a low altitude experimental group 4. Through Multi - DopX transcranial doppler ultrasound monitor (Germany), and QL software application system to measure the before and after inhaled CO2 bilateral middle cerebral artery of cerebral blood flow velocity and respiratory CO2 partial pressure, at the end of the calculation of cerebrovascular reactivity. Enzyme linked immunosorbent (ELISA) method was recruited to determine the plasma levels of eNOS, the plasma NO level was determined by nitrate reduction method. Results Localized high altitude Tibetans cerebrovascular reactivity, plasma NO,plasma eNOS have no difference with the Han Chinese who moved from low altitude 第 第 PAGE 4 页，共 32 页 (Tibetan CVR2.05 ± 0.29, the han nationality 1.93 ±0.21 (P 0.05); the Tibetan NO53.81 ± 3.01, the han