紫甘薯花色苷的提取 纯化及肝损伤保护功能的研究-study on the extraction and purification of anthocyanins from purple sweet potato and the protective function of liver injury.docxVIP

IV IV Based on the above process, a systematic production process was obtained. Under this condition, the colour value of PSPPs reached 27.7. The hepa-protective effect in vivo: using CCl4 to ind uce acute and chemical liver injury model, contrasting the ALT and AST indictors in serum among the control group, PSPPs low, medium and high dose group and the model group, the conclusion has been reached that PSPPs had some preventive effects on acute and chemical liver injury. PSPPs medium and high dose group could significantly reduce the level of Alanine Transaminase (ALT), and the effect of medium dose group (P=0.011) was better than high dose group (P=0.021). The optimal dose can be speculated is the medium dose of 455mg/Kg, and high dose may have some side effects. Compared with model group, the inhibition of PSPPs high dose on Aspartate Aminotransferase (AST) was significantly different while the surpression of PSPPs low and medium dose was limited. The protection of hepatocytes injured by H2O2 : the Glutathione Peroxidase (GSH-Px) activity of PSPPs medium and high dose group was higher than the oxidative damage group, and the difference was statistically significant (P0.01); the Superoxide Dismutase (SOD) activity of midium and high dose group and the model group had significant difference (P0.01); the Maleic Dialdehyde (MDA) level of PSPPs medium and high dose group was lower than the model group, and the difference was statistically significant (P0.01). Keyword ： Purple sweet potato;anthocyanins;extraction;purification;hepa-protective effect 烟台大学学位论文原创性声明和使用授权说明 原创性声明 本人郑重声明： 所呈交的学位论文，是本人在导师的指导下，独立进行研究工 作所取得的成果。除文中已经注明引用的内容外，本论文不含任何其他个人或集体 已经发表或撰写过的作品或成果。对本文的研究做出重要贡献的个人和集体，均已 在文中以明确方式标明。本声明的法律结果由本人承担。 论文作者签名： 日期： 年 月 日 学位论文使用授权说明 本人完全了解烟台大学关于收集、保存、使用学位论文的规定，即：按照学校 要求提交学位论文的印刷本和电子版本；学校有权保存学位论文的印刷本和电子版， 并提供目录检索与阅览服务；学校可以采用影印、缩印、数字化或其它复制手段保 存论文；在非保密的论文范围内，学校可以公布论文的部分或全部内容。 （保密论文在解密后遵守此规定） 论文作者签名： 导师签名： 日期： 年 月 日 烟台大学硕士学位论 烟台大学硕士学位论文 55 55 版权声明 任何收存和保管本论文各种版本的