急腹症Ⅲ号对呼吸机相关性肺炎大鼠小肠形态影响.docVIP

急腹症Ⅲ号对呼吸机相关性肺炎大鼠小肠形态影响 　　[摘要] 目的 ?^察急腹症Ⅲ号对呼吸机相关性肺炎模型大鼠小肠形态的影响，并探讨其作用机制。 方法 雄性SD大鼠100只，随机分为五组：急腹症Ⅲ号组（VAP模型，急腹症Ⅲ号灌胃）、谷氨酰胺组（VAP模型，谷氨酰胺颗粒灌胃）、急腹症Ⅲ号联合谷氨酰胺组（VAP模型，急腹症Ⅲ号及谷氨酰胺颗粒灌胃）、安慰剂组（VAP模型，生理盐水灌胃）、空白对照组（生理盐水灌胃）。术后第5天收集尿液，采用L/M（乳果糖/甘露醇法）评价肠黏膜通透性，术后第6天处死大鼠取小肠进行病理检测。 结果 安慰剂组L/M较空白对照组明显增大（P0.05），且均高于安慰剂组（P0.05）。空白组黏膜厚度与急腹症Ⅲ号组及联合组相似（P0.05），但高于谷氨酰胺组（P0.05）。联合组全层厚度高于安慰剂组（P0.05）。 结论 急腹症Ⅲ号可减少呼吸机相关性肺炎大鼠模型肠黏膜通透性增加，减少小肠黏膜结构的破坏，保护肠黏膜结构。 　　[关键词] 急腹症Ⅲ号；呼吸机相关性肺炎；肠道通透性；黏膜结构 　　[中图分类号] R259 [文献标识码] A [文章编号] 1673-9701（2018）03-0030-04 　　Effect of acute abdomen No.Ⅲ on small intestine morphology of rats with ventilator-associated pneumonia 　　WANG Ruiming 　　Department of Emergency， Hangzhou Hospital of TCM， Hangzhou 310007， China 　　[Abstract] Objective To observe the effect of acute abdomen No.Ⅲ on small intestine morphology in rats with ventilator-associated pneumonia and to explore its mechanism. Methods A total of 100 male SD rats were randomly divided into 5 groups. Acute abdomen No.Ⅲ group： VAP model， acute abdomen No.Ⅲ gavage； glutamine group： VAP model， glutamine granules gavage； acute abdomen No.Ⅲ combined with glutamine group： VAP model， acute abdomen No.Ⅲ and glutamine granules gavage； placebo group： VAP model， normal saline gavage； blank control group： normal saline gavage. Urine was collected on the 5th day after surgery. L/M （lactulose/mannitol method） was used to evaluate the intestinal mucosal permeability. The rats were sacrificed on the 6th day after surgery to collect the small intestine for pathological examination. Results L/M in placebo group was significantly higher than that in blank control group（P0.05）， and were all higher than that in the placebo group（P0.05）. The mucosal thickness in the blank group was similar to that in the acute abdomen No.Ⅲ group and the combined group（P0.05）， but higher than glutamine group（P0.05）. The total thickness in the combined group was higher than that in the placebo group（P0.05）. Conclusion Acute abdomen No.Ⅲ can reduce the incre