急性冠状动脉综合征患者外周血单核细胞核因子κB活性变化临床探讨.docVIP

急性冠状动脉综合征患者外周血单核细胞核因子κB活性变化临床探讨 　　［摘 要］ 目的 探讨急性冠状动脉综合征(acute coronary syndro me, ACS)患者外周血单核细胞核因子-κB(nuclear factor-kappa B, NF-κB)活性的变化及 其临床意见。方法 分别采用免疫组织化学染色法和夹心酶联免疫吸附法 测定54例ACS，42例稳定型冠心病(SCHD)患者及36例对照组的外周血单核细胞NF-κB活性和 血浆可溶性细胞间黏附分子-1(sICAM-1)水平，并分析NF-κB活性与sICAM-1浓度相关性。结果 ASC组NF-κB活性和sICAM-1水平显著高于稳定型冠心病组和对照组( P＜0.01)，而且ACS血NF-κB活性与sICAM-1水平显著正相关。但SCHD组NF-κB活性和sIC AM-1水平与对照组比较，差异无显著性意义。结论 急性冠状动脉综合征 外周血单核细胞核因子-κB活性明显升高，NF-κB是动脉粥样斑块不稳定的标志，并可能通 过上调sICAM-1表达而促发ACS。 　　［关键词］ 急性冠状动脉综合征；核因子-κB；可溶性细胞间黏附分 子-1 　　中图分类号：R541.4 　　文献标识 码：A 　　文章编号：1009-816X(2007)02-0299-03 　　The Clinical Significance of Changes of the Activity of Peripheral Bl ood Mononuclear Cell NF-κB in Acute Coronary Syndrome. TANG Xiao-fan g, WANG Qiang, WANG Yin. Zhejiang Hospital, Zhejiang 310013, China 　　［Abstract］ Objective To investigate the clinical significance of changes of the activity of peripheral blood mononuclear cell NF-κB in acute coronary syndrome (ACS). Methods Enzyme-linked immunosorbent ass ay was used to measure the plasma soluble intercellular adhesion molecule-1 (sIC AM-1) and immunohistochemical method was used to measure activity of NF-κB in p e ripheral blood mononuclear cell in 58 patients with ACS, 48 stable coronary hear t disease (SCHD) and 30 normal control subjects. At the same time linear correl ation analysis was used to analyze the correlation between the level of sICAM-1 and the level of activity of NF-κB. Results The level of activi ty of NF-κB in peripheral blood mononuclear cells and the plasma sICAM-1 cocent ration were significantly higher in patients with ACS than in the controls and i n patients with SCHD. The level of plasma sICAM-1 was positively correlated with activity of NF-κB in peripheral blood mononuclear cells. There was no signific ant difference in levels of activity of NF-κB in peripheral blood mononuclear c ell and the plasma sICAM-1 concentration between the controls and the patients w ith SCHD. Conclusions The activity of NF-κB in peripheral bloo