枸杞多糖对小鼠脑缺血再灌注损伤保护作用及其抑制NF―κB,TNF―α,IL―6 和 IL―1β表达机制.docVIP

枸杞多糖对小鼠脑缺血再灌注损伤保护作用及其抑制NF―κB,TNF―α,IL―6 和 IL―1β表达机制 　　[摘要]探讨枸杞多糖（Lycium barbarum polysaccharides，LBP）对缺血再灌脑损伤小鼠的保护作用及其可能的机制。采用颈总动脉栓线造成大脑中动脉缺血，缺血2 h后将栓线拔出以实现大脑中动脉血流再灌注，形成小鼠短暂性大脑中动脉阻塞（transient middle cerebral artery occlusion，tMCAO）模型，观察LBP（25，50，100 mg?kg-1）对小鼠脑梗死范围，脑含水量，神经症状的影响；采用HE染色观察LBP对小鼠脑组织神经细胞的影响，Western blot法检测缺血大脑皮层NFκB p65蛋白的表达，ELISA法检测血清中TNFα，IL6和IL1β的水平。结果显示，LBP对缺血再灌注小鼠神经症状有明显的改善作用，能明显降低脑梗死范??和脑含水量。脑组织病理切片也证实了LBP对神经细胞的保护作用。Western blot结果显示，小鼠缺血再灌后缺血侧大脑皮层p65蛋白水平明显增高，LBP能显著降低p65蛋白水平。ELISA结果显示，LBP能显著降低缺血再灌后TNFα，IL6和IL1β的生成。表明LBP对缺血再灌注小鼠脑损伤有明显保护作用，该作用可能与其抑制NFκB和炎症反应有关。 　　[关键词]枸杞多糖； 脑缺血； 核因子κB； 肿瘤坏死因子α； 白细胞介素6； 白细胞介素1β 　　Protective effects of LBP on cerebral ischemia reperfusion injury in mice and 　　mechanism of inhibiting NFκB， TNFα， IL6 and IL1β 　　GE Jianbin1，2， LU Hongjian1， SONG Xinjian1， LI Mei2， CHEN Dandan3， WU Feng3* 　　（1 The Second People′s Hospital of Nantong， Nantong 226002， China； 　　2 Department of Pharmacology， College of Pharmaceutical Science， Soochow University， Suzhou 215123， China； 　　3 Department of Pharmacology， Nantong University， Nantong 226001，China） 　　[Abstract]To observe the protective effects of Lycium barbarum polysaccharides （LBP） on cerebral ischemia reperfusion injury in mice and explore its mechanism Common carotid artery thread was used to cause middle cerebral artery ischemia， and the thread was taken out after 2 h ischemia to achieve cerebral ischemia reperfusion injury in mice Therefore， the transient middle cerebral artery occlusion （tMCAO） models were established to observe the effects of LBP （25，50， 100 mg?kg-1） on neurological outcome， infarct size and water contents HE staining was used to observe its effects on neurocytes of cerebral tissues in mice Western blotting was used to evaluate the protein expression levels of NFκB p65 ELISA was used to evaluate the levels of TNFα， IL6 and IL1β in the serum According to the results， LBP markedly improved neurologic deficits， and decreased infarct size and water conten