温度对猕猴桃花青素含量及其合成基因AdANS影响.docVIP

温度对猕猴桃花青素含量及其合成基因AdANS影响 　　 摘要：为明确‘红阳’猕猴桃果肉中花青素含量在季节性温度下的合成变化情况及花青素合成酶基因ＡｄＡＮＳ表达的变化，以‘红阳’主产区四川蒲江和湖北武汉的‘红阳’猕猴桃为研究对象，测定其花青素的含量并克隆花青素合成基因ＡｄＡＮＳ，并对其转录水平变化进行检测。结果表明，较高的季节性温度对果肉花青素的含量有抑制作用，在温度适宜的蒲江，ＡｄＡＮＳ基因的表达水平与花青素积累变化相一致，而在高温的武汉其表达水平受到明显的抑制。 　　关键词：‘红阳’猕猴桃；花青素；温度波动；ＡｄＡＮＳ基因 　　中图分类号：Ｓ６６３．４文献标识码：Ａ文章编号：0439－８114（２０14）09－2070-05 　　 　　Effects of Temperature on Anthocyanin Content and Anthocyanin Synthase (AdANS) 　　of ‘Hongyang’ Kiwifruit 　　 　　LI Wen-bin1,2,3,LIU Yi-fei4,PENG Ming1 　　(1.Institute of Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 　　2.Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Scienes, Wuhan 430074, China; 3.University of Chinese of Academy of Sciences, Beijing 100039, China; 4.Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China) 　　 　　Ａｂｓｔｒａｃｔ：To illustrate the changes of anthocyanin concentration and anthocyanin synthase under seasonal fluctuated temperature, the concentration of anthocyanin of ‘Hongyang’ kiwifruit from Pujiang, Sichuan and Wuhan, Hubei was measured under different seasonal temperatures. Anthocyanin synthase (AdANS) was cloned. Quantitative real time PCR (RT-PCR) was used to detect the expression of AdANS during fruit development. Result showed that seasonal high temperature had negative regulation on anthocyanin concentration in flesh. The expression level of AdANS was positively cor related with anthocyanin synthesis in Pujiang with suitable temperature and was inhibited greatly in Wuhan with high temperature. 　　Ｋｅｙ ｗｏｒｄｓ： ‘Hongyang’ kiwifruit; anthocyanin; temperature fluctuation; AdANS 　　猕猴桃（Ａｃｔｉｎｉｄｉａ ｃｈｉｎｅｎｓｉｓ Ｐｌａｎｃｈ）驯化栽培至今已有１００余年的历史［１］，因其具有独特的风味和丰富的营养而受到广大消费者的喜爱。尤其是‘红阳’猕猴桃以其果实中心有放射状红色条纹，富含花青素而成为近年来消费者的新宠。但该品种不耐夏季高温干旱，特别是果肉红色易受夏季温度和湿度的影响，田间观察发现，在夏季温度较高的武汉种植的‘红阳’猕猴桃果肉的颜色非常淡，严重影响果实的商品价值［２］。 　　温度是影响