生化检验标本放置时间对结果影响.docVIP

生化检验标本放置时间对结果影响 　　作者单位：510540 广州友好医院（詹燕华）；广东省茂名市中医院（周世锋） 　　通讯作者：詹燕华 　　【摘要】 目的 观察空腹静脉血标本静置时间对常规生化检验结果的影响。方法 随机抽取134例住院患者早晨空腹静脉血标本，每例血标本分别放置于3个试管内，分别在1 h、2 h、3 h分离血清，检测血糖、尿素氮、肌酐、钾、钠、氯、谷丙转氨酶、总胆红素、直接胆红素9个项目。结果 血糖采血后2 h与3 h、氯采血后3 h与1 h检测结果比较差异有统计学意义（P0.05）。结论 测空腹血糖采血时应注明时间，1 h内分离血清进行检测；氯应在采血后2 h内分离血清进行检测。 　　【关键词】 空腹血标本； 静置时间； 生化检验 　　Impact of laying-aside time of blood sample on routine biochemical laboratory results ZHAN Yan-hua, ZHOU Shi-feng. Guangzhou Youhao Hospital, Guangzhou 510540, China 　　【Abstract】 Objective To observe the impact of laying-aside time of fast venous blood samples on the laboratory results of routine biochemical items.Methods A total of 134 patients were selected randomly and their fasting blood samples were collected into 3 different test tubes. Serum was separated 1 h, 2 h, and 3 h later respectively. Altogether 9 items including blood glucose, urea nitrogen, creat inine, potassium, sodium, chlorine, glutamic-pyruvic transaminase, total bilirubin, and direct bilirubin were detected.Results In terms of the detected blood glucose concentration results, there was statistcal significance between 2 h and 3 h of blood samples laying aside. In terms of blood chlorine, there was statistical significance between 3 h and 1 h later the blood samples being collected （P0.05）.Conclusion If blood sample was prepared for detecting fasting blood glucose, it should be marked the time of being collected. The blood sample should be detected within 1 h after collection. For blood chloride detection, it is better to detect it within 2 h after sample collection. 　　【Key words】 Fasting blood samples； Time of laying aside; Biochemical detection 　　 在临床检验过程中，有时标本采集后会得不到及时检测，如仪器故障、试剂短缺、停水停电、以及远程检验需要标本的邮寄等等。对于血清标本放置多长时间检测不会超出实验室允许范围，有关临床化学检验的参考书中所提及内容不尽一致。为了探讨护士采血与检验间隔时间对常规生化结果的影响，笔者于2010年8月6日随机对134例住院患者采取统一采血时间，分别观察同一标本间隔不同时间检验，对结果的差异性进行自身对比，现将结果报告如下。 　　1 资料与方法 　　1.1 一般资料 随机选取2010年8月6日134例本院需空腹采血做常规生化检验的住院患者作为研究对象，血清标本无溶血、黄疸、脂血，其中内科68例，外科60例，妇产科6例；男76例，女58例；年龄22