玉竹提取物对D半乳糖衰老小鼠模型学习记忆影响.docVIP

玉竹提取物对D半乳糖衰老小鼠模型学习记忆影响 　　【摘 要】:目的:观察玉竹提取物对D-半乳糖(D-gal)衰老模型小鼠学习记忆能力及抗氧化能力的影响;方法:5%D-gal皮下注射建立衰老小鼠模型,同时灌胃给予玉竹提取物(extract of Polygonatum odoratum,E-PAOA),正常对照组和模型组给予等体积生理盐水。用Morris水迷宫实验检测小鼠的学习记忆能力,生化法检测小鼠脑组织超氧化物歧化酶SOD活力及丙二醛MDA含量;结果:与正常对照组相比,模型组小鼠学习记忆能力降低,脑组织SOD活力下降,MDA含量增加。灌胃给予E-PAOA后,小鼠上述变化明显改善;结论:E-PAOA可改善D-gal衰老模型小鼠的学习记忆能力,具有促进智力和延缓衰老的作用,其机制可能E-PAOA与具有抗氧化能力有关。 　　【关键词】:玉竹提取物;D-半乳糖;学习记忆 　　【中图分类号】R284.2【文献标识码】 B【文章编号】1007-8517(2009)01-0006-02 　　 　　Effects ofextract of Polygonatum odoratum on learning and memory function of aging miceinduced by D-galactose 　　 　　 Abstract:Objective: To observe effects of the extract of Polygonatum odoratum (E-PAOA)on learning and memory function and anti-oxidative ability of aging model mice induced by D-galactose. Methods: Senile model mice were established by D-galactose hypodermic injection for 8 weeks. E-PAOA had been administered to three therapy groups. All the mice of different groups were tested with Morris water maze. Then the mice were killed and biochemically assayed of superoxide dismutase SOD and malondialdehyde MDA in the brain. Results: The model mice showed worse ability in learning and memory in comparison with control mice. TheSOD activity in the brain decreased and the MDA content increased in model rats in comparison with control. E-PAOA significantly improved the changesmentioned above. Conclusion E-PAOA improved the learning and memory dysfunction in aging model mice by modulation of the anti-oxidative ability. 　　 Key words: the extract of Polygonatum odoratum;D-galactose; learning and memory; 　　 　　阿尔茨海默病(Alzheimer disease,AD)是老年人常见的一种慢性进行性神经系统退行性疾病[1]。D-半乳糖 (D-galactose,D-gal)模型作为衰老模型之一,已被证实与人类自然衰老有类似的生化改变、细胞的退行性变、动物记忆巩固障碍和再现障碍[2]等。玉竹(Polygonatum odoratum MilI Druce PO )为百合科黄精属植物,味甘平,性微寒,具有滋阴润燥,养胃生津之功,现代药理学研究发现,玉竹中含有甾体皂甙、黄酮、多糖、生物碱、强心甙等多种成分[3]。本实验采用连续8周皮下注射D-gal的方法建立衰老小鼠模型,观察玉竹提取物对其学习记忆及抗氧化能力的影响并探讨其作用机制,为玉竹用于抗衰老和防治 AD提供实验依据。 　　 　　1 材料与方法 　　 　　1.1 材料 　　1.1.1 实验动物:昆明种小鼠,雌性,体重(222g),为本学院自己饲养。 　　1.1.2 药