破血化瘀填精补髓法对实验性脑出血大鼠血肿周围脑组织GSK―3β信号通路作用研究.docVIP

破血化瘀填精补髓法对实验性脑出血大鼠血肿周围脑组织GSK―3β信号通路作用研究 　　摘要：目的 探讨破血化瘀、填精补髓法对大鼠脑出血血肿周围组织GSK-3β信号通路表达的影响。方法 应用WISTAR大鼠复制脑出血大鼠模型，观察破血填精法在改善脑出血大鼠神经功能缺损及大鼠血肿周围组织GSK-3β信号通路表达情况。结果 与模型组相比，阳性药和受试药各组大鼠在给药1d和3d后能明显改善大鼠神经系统体征，降低神经功能缺损评分；在给药第3、7、14d，与模型组比较，受试药高剂量和中剂量均明显增加了损伤脑区GSK-3β信号通路表达数量（P0.05或P0.01，P0.05）。结论 破血填精法方剂能明显改善脑出血大鼠神经功能缺损；其作用机制可能为调高脑出血血肿周围损伤组织中GSK-3β信号通路表达有关。 　　关键词：脑出血；GSK-3β；破血化瘀；填精补髓 　　Abstract：Objecttive To investigate the effect of decoction of Poxue and Tianjing on the expression of brain derived neurotrophic factor GSK-3βand its receptor to the perihematomal tissue in experimental ICH Rats.Methods Using WISTAR rats as the model rats of cerebral hemorrhage.It is designed to observe the effect of the behavioristic index that how the Decoction of Poxue and Tianjing could improve the neurological deficit of the rats.There is also a experiment which is designed to observe the protein expression of GSK-3β.Results Compared with the model control group，the positive drug group and the investigational drug groups ratsneurological signs are improved after 1 and 3 daysadministration.At the third，the seventh and fourteenth day of administration，compared with the model group，high and medium-dose group damaged brain area GSK-3βquantity are clearly increased（P0.05 or P0.01，P0.05）. Conclusion The experiment has proved that the intracerebral hemorrhage ratsneurological deficit has been evidently improved by the Poxue and Tianjing prescription.And it has been proved from the molecular level that the mechanism might be increasing the expression of GSK-3βto the perihematomal tissue in experimental ICH rats. 　　Key words：Intracerebral hemorrhage；GSK-3β；Poxue and Tianjing 　　近十年来对脑出血后脑损伤发生机制的认识得到快速的发展。动物实验研究已经证实了作为出血结果的初期的机械性创伤、凝血衍生因子和肿块效应的作用。中医药治疗脑出血的基础实验研究也在逐年开展，目前在促进血肿吸收，减轻脑水肿，提高实验大鼠神经功能评分方面得到了证实。同时中医药在减轻脑出血后自由基损伤、减轻炎性反应、抑制细胞凋亡也有大量的文献报道。本研究力求从蛋白水平证明其作用机制，通过运用破血填精法中药汤剂针对实验性脑出血大鼠GSK-3β信号通路表达的影响。 　　1资料与方法 　　1.1一般资料 清洁级体重为250～300g Wistar大鼠，由中国医学科学院中国协和医科大学实验动物研究所提供，取雌雄各50%。破血化瘀、填精补髓法方剂：（瓜蒌2