胃癌患者手术前后血清胸苷激酶1水平变化临床研究.docVIP

胃癌患者手术前后血清胸苷激酶1水平变化临床研究 　　[摘要] 目的 探讨检测血清胸苷激酶（TK1）水平对胃癌诊断及手术效果评估的意义。方法 采用免疫印迹-增强化学发光法检测35例健康查体者和68例胃癌患者血清TK1水平，比较43例胃癌患者根治术前后血清TK1水平。结果胃癌患者血清TK1水平明显高于正常对照组 （P0.01）；血清TK1水平与胃癌患者性别、年龄无相关，与胃癌患者临床分期、细胞分化程度相关；Ⅳ期明显高于Ⅲ期、Ⅲ期明显高于Ⅱ期、Ⅱ期明显高于Ⅰ期、Ⅰ期明显高于正常对照组，两两比较差异有统计学意义（P0.01～0.05），肿瘤细胞分化程度较差胃癌患者血清TK1水平明显高于分化程度较好胃癌患者；术后胃癌患者血清TK1水平明显低于术前（P0.01）。结论 检测血清TK1水平对胃癌诊断、预测病情进展、疗效等有较大的临床价值。 　　[关键词] 胸苷激酶1； 胃癌； 手术治疗 　　[中图分类号] R735.2 [文献标识码] B [文章编号] 1673-9701（2014）27-0151-03 　　[Abstract] Objective To evaluate the use of Thymidine Kinase 1（TK1） in serum as proliferating tumor marker for gastric carcinoma and monitoring marker for the response to operation. Methods Serum level of TK1 expression were detected with sensitive chemiluminescence dot- blot assay in were available to detect the level of serum TK1 in 35 cases of normal healthy subject and 68 cases of patients with gastric carcinoma， compared with 43 cases of patients with gastric carcinoma pre-and post-operation. Results The level of serum TK1 were elevated in all subgroups of patients with gastric carcinoma compared to the normal healthy subject （P0.01）， serum TK1 was correlated with TNM stage and histological grade， and not with sex and age； the serum TK1 level in stage Ⅳwas higher in comparison with stage Ⅲ， in stage Ⅲit was higher than that in stage Ⅱ， in stage Ⅱit was higher than that in stage Ⅰ， and in stage Ⅰit was higher than that in normal healthy subject （P0.01-0.05）； Being lower in the well differentiated and moderately differentiated than the poorly differentiated （P0.01-0.05）， the level of serum TK1 was higher in the cases of poorly differentiated adenocarcinoma， signet ring cell carcinoma and mucoid adenocarcinoma than those in the cases of well differentiated and moderately differentiated adenocarcinoma （P0.05）， the serum TK1 levels remarkably reduced in patients with gastric carcinoma after operation （P0.01）. Conclusion Detection the level of serum TK1 may be larger clinical value for cancer diagnosis， predict disea