高脂饮食对大鼠肝脏PGC1αTRB3Akt影响.docVIP

高脂饮食对大鼠肝脏PGC1αTRB3Akt影响 　　[摘要] 目的 观察高脂饮食对大鼠肝脏PGC-1α、TRB3、Akt蛋白水平及血浆脂联素的影响，探讨其引起胰岛素抵抗的机制。方法 将24只雄性Wistar大鼠按照体重随机分为两组，分别给予普通饮食和高脂饮食，22周后处死大鼠，测量体重、体脂重量，取血检测血脂、血糖、胰岛素和脂联素；取肝脏检测PGC-1α、TRB3、Akt蛋白水平。结果 与对照组相比，高脂饮食组大鼠空腹血糖、胰岛素和胰岛素抵抗指数分别增加27.1%（P＞0.05）、27.3%（P＜0.05）和68.8%（P＜0.01），血浆中脂联素含量降低，空腹血清TC、HDL-C、LDL-C及TG水平和正常对照组大鼠无显著性差异；高脂饮食组大鼠肝脏PGC-1α蛋白水平上升（P＜0.05），TRB3蛋白水平上升（P＜0.05），Akt蛋白水平下降（P＜0.05）。 结论 高脂饮食可诱导大鼠产生肥胖和胰岛素抵抗，可能和PGC-1α/TRB3/Akt信号通路有关。 　　[关键词] 高脂饮食；PGC-1α；TRB3；Akt；胰岛素抵抗 　　[中图分类号] R589.2；R575.5 [文献标识码] B [文章编号] 1673-9701（2011）35-14-03 　　 　　Effects of High-fat Diet on PGC-1α,TRB3,Akt Proteins in Rat Liver Tissue 　　BIAN Dezhi1 ZHAO Qingsong2 SUI Ping1 ZHANG Fan1 LI Lei1 　　1.Clinical College of Jining Medical University，Jining 272013，China； 2.Endocrinology Department of the Affiliated Hospital to Jining Medical University，Jining 272029，China 　　 　　[Abstract] Objective To observe the effects of high-fat diet on plasma adiponectin and PGC-1 alpha，TRB3，Akt protein levels in rat liver，and discuss the mechanism on insulin resistance. Methods Twenty-four male Wistar rats according to weight were randomly divided into two groups，separately given ordinary diet and high-fat diet for 22 weeks；Then measured body weight，body fat weight，collected the blood and tested blood fat and blood glucose，insulin and adiponectin；Collected the liver tissues to detect PGC-1 alpha，TRB3 and Akt protein levels. Results Comparing with the control group，fasting blood glucose，fasting insulin and HOMA-IR index of high-fat diet group were increased by 27.1%（P＞0.05），27.3%（P＜0.05） and 68.8%（P＜0.01），plasma adiponectin level was reduced；Fasting serum TC，HDL-C，LDL-C and TG levels had no significant difference with the control group. In high-fat diet group，rat liver PGC-1α protein level was increased（P＜0.05），TRB3 protein level was increased（P＜0.05），Akt protein level was reduced（P＜0.05）. Conclusion High fat diet can induce rat to be obesity and insulin resistance，and PGC-1α/TRB3/Akt signaling pathway mig